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Commit 986d4755 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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added metatadata + data for MSA dissection SD/LD

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- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD)
- start 5 days after emergence (DAE)
- remove the leaves surrounding the main shoot apex (MSA) with a blade
- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera
- score developmental stage according to Waddington et al. (1983)
- count spikelet meristems (include those that have developet into floret meristems or florets) on the MSA
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Scoring of the development according to:
Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
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......@@ -20,5 +20,5 @@ genotypes:
**plants**
- SD: sample names include "SD"
- LD: sample names include "LD"
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- SD: plant 1 - 204
- LD: plant 205 - 342
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