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Commit cb35c300 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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**Allelism test** **Allelism test**
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**Allelism test*
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Complementation crosses were performed to confirm that *lwd1* and *eam7* are allelic, which are both recessive mutations. For this, homozygous *lwd1* mutants *lwd1-26*, *lwd1-390*, and *lwd1-402* were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If *lwd1* and *eam7* are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD. Complementation crosses were performed to confirm that *lwd1* and *eam7* are allelic, which are both recessive mutations. For this, homozygous *lwd1* mutants *lwd1-26*, *lwd1-390*, and *lwd1-402* were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If *lwd1* and *eam7* are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD.
**study overview** **study overview**
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parent plants (lwd1 mutants, BW(Ppd-H1,eam7), BW(Ppd-H1), GP-fast) parent plants (lwd1 mutants, BW(Ppd-H1,eam7), BW(Ppd-H1), GP-fast)
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parent plants (*lwd1* mutants, BW(Ppd-H1,eam7), introgression line background BW(Ppd-H1), mutant background GP-fast)
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-> assay: complementation_crosses -> assay: complementation_crosses
output: F1 plants output: F1 plants
...@@ -13,7 +21,11 @@ Complementation crosses were performed to confirm that *lwd1* and *eam7* are all ...@@ -13,7 +21,11 @@ Complementation crosses were performed to confirm that *lwd1* and *eam7* are all
output: F1 genotype information output: F1 genotype information
-> assay: plant_phenotyping -> assay: plant_phenotyping
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output: F1 flowering data output: F1 flowering data
Results can be found in Figure 6 and Supplemental Figure S8. Results can be found in Figure 6 and Supplemental Figure S8.
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output: F1 flowering data
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