assays/complementation_crosses/protocols/crossing_plants.md

@@ -7,5 +7,5 @@
 **Crossing:**
 - emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0)
 - pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross
-- cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds
+- cover the spike with a paper bag
 - harvest seeds from each spike / each cross individually when they are completely dry
\ No newline at end of file

assays/complementation_crosses/protocols/score_crosses.md

+## Score successful crosses
+
+- all crossed spikes were covered with a paper bag
+- number of pollinated florets per spike were documented
+- when seeds were ripe: count how many of the polinated florets developed into seeds
\ No newline at end of file

assays/embryo_transformation/protocols/cloning_trafo_protocol.md

@@ -15,9 +15,6 @@
 
 - GP-fast plants were cultivated in long-day conditions (16h day / 8 night) in control temperature (20°C / 16°C)
 - immature embryos were used for transformation according to Hensel et al. (2009)
-- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
-- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
- 
 
 
 **References:**

assays/embryo_transformation/protocols/test_hygromycin.md

+## Test regenerated plants for successful transformation
+
+- DNA was extracted from regenerated plants using QIAGEN_DNeasy-Plant-Mini protocol
+- successful insertion of the transformation vector into the genome was tested by PCR using the primers Hyg-156 (5'-ACGCACAATCCCACTATCC-3') and Hyg-047 (5'-GTGTCGTCCATCACAGTTTG-3')
+