add blots to S5_2 + readme

.gitattributes

@@ -27,8 +27,6 @@ assays/S4_8_Growth-curves-of-WT-Adea373-and-Adea442/dataset/.gitkeep filter=lfs
 assays/S4_8_Growth-curves-of-WT-Adea373-and-Adea442/dataset/Growth[[:space:]]curve_WT-Adea373-Adea442.pzf filter=lfs diff=lfs merge=lfs -text
 assays/S5_1_Genomic-map-of-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
 assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
-assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-09[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
-assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/2025-01-13[[:space:]]Chemiluminscence.tif filter=lfs diff=lfs merge=lfs -text
 assays/S5_3_Growth-curves-of-WT-and-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
 assays/S5_3_Growth-curves-of-WT-and-Adea456/dataset/Growth[[:space:]]curve_WT-Adea456.pzf filter=lfs diff=lfs merge=lfs -text
 assays/S5_4_Epifluorescence-microscopy-of-Adea126-and-Adea456/dataset/.gitkeep filter=lfs diff=lfs merge=lfs -text
@@ -70,3 +68,7 @@ assays/S4_1_Genomic-map-of-Adea400/dataset/Map_Adea400.jpg filter=lfs diff=lfs m
 assays/S4_6_Knock-out-strategy-for-T7RNAP/dataset/Map_Adea442.jpg filter=lfs diff=lfs merge=lfs -text
 assays/S5_1_Genomic-map-of-Adea456/dataset/Map_Adea456.jpg filter=lfs diff=lfs merge=lfs -text
 assays/S4_6_Knock-out-strategy-for-T7RNAP/dataset/Thumbs.db filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-20[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BamHI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-22[[:space:]]Chemiluminescence.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-09[[:space:]]SYBR[[:space:]]Safe[[:space:]]stained[[:space:]]gel.tif filter=lfs diff=lfs merge=lfs -text
+assays/S5_2_Verification-of-insertion-by-Southern-Blot/dataset/BclI-SpeI[[:space:]]Southern[[:space:]]-[[:space:]]2025-01-13[[:space:]]Chemiluminscence.tif filter=lfs diff=lfs merge=lfs -text

README.md

@@ -58,7 +58,7 @@ The [Assays](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_
 
 ### Chapter 5: Analysis of gene expression strength
 * [S5_1_Genomic map of Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_1_Genomic-map-of-Adea456?ref_type=heads) // **Fig. 5A**
-* [S5_2_Verification of insertion by Southern Blot](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_2_Verification-of-insertion-by-Southern-Blot?ref_type=heads) // **Fig. 5B**
+* [S5_2_Verification of insertion by Southern Blot](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_2_Verification-of-insertion-by-Southern-Blot?ref_type=heads) // **Fig. 5B** + supporting material **S1 Figure**
 * [S5_3_Growth curves of WT and Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_3_Growth-curves-of-WT-and-Adea456?ref_type=heads) // **Fig. 5C**
 * [S5_4_Epifluorescence microscopy of Adea126 and Adea456](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_4_Epifluorescence-microscopy-of-Adea126-and-Adea456?ref_type=heads) // **Fig. 5D**
 * [S5_5_Quantification of fluorescent cells](https://git.nfdi4plants.org/lena.kroeninger/2025_Expression_System_Adeanei/-/tree/main/assays/S5_5_Quantification-of-fluorescent-cells?ref_type=heads) // **Fig. 5E**