Ningning Zhang, Benedikt Venn, Catherine E Bailey, Ming Xia, Erin M Mattoon, Timo Mühlhaus, Ru Zhang, Moderate high temperature is beneficial or detrimental depending on carbon availability in the green alga Chlamydomonas reinhardtii, Journal of Experimental Botany, 2023;, erad405, https://doi.org/10.1093/jxb/erad405 Titel anhand dieser DOI in Citavi-Projekt übernehmen
## Project outline
<i>Chlamydomonas reinhardtii</i> CC-1690 was grown in bioreactors. When the cells reached a non-stationary density (2E+6 cells / ml), the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In [Zhang et al 2022](https://doi.org/10.1038/s42003-022-03359-z) samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples).
<i>Chlamydomonas reinhardtii</i> CC-1690 was grown in bioreactors. When the cells reached a non-stationary density (2E+6 cells / ml), the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In [Zhang et al 2022](https://doi.org/10.1038/s42003-022-03359-z) samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples).
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@@ -88,4 +94,4 @@ Heatmaps, PCA, and functional sets figures were created using Plotly.NETv4.0.0 (
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@@ -88,4 +94,4 @@ Heatmaps, PCA, and functional sets figures were created using Plotly.NETv4.0.0 (
- Zhang, N., Mattoon, E.M., McHargue, W. et al. Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii. Commun Biol 5, 460 (2022). https://doi.org/10.1038/s42003-022-03359-z
- Zhang, N., Mattoon, E.M., McHargue, W. et al. Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii. Commun Biol 5, 460 (2022). https://doi.org/10.1038/s42003-022-03359-z
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- featureCounts: Liao Y, Smyth GK, Shi W. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2014 Apr 1;30(7):923-30.
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- May P, Christian JO, Kempa S, Walther D. ChlamyCyc: an integrative systems biology database and web-portal for Chlamydomonas reinhardtii. BMC Genomics. 2009 May 4;10:209. doi: 10.1186/1471-2164-10-209. PMID: 19409111; PMCID: PMC2688524.
