Update README.md

README.md

 # Carbon Availability Transcriptomics
 
+## Publication
+
+Ningning Zhang, Benedikt Venn, Catherine E Bailey, Ming Xia, Erin M Mattoon, Timo Mühlhaus, Ru Zhang, Moderate high temperature is beneficial or detrimental depending on carbon availability in the green alga Chlamydomonas reinhardtii, Journal of Experimental Botany, 2023;, erad405, https://doi.org/10.1093/jxb/erad405 Titel anhand dieser DOI in Citavi-Projekt übernehmen
+
+## Project outline
+
 <i>Chlamydomonas reinhardtii</i> CC-1690 was grown in bioreactors. When the cells reached a non-stationary density (2E+6 cells / ml), the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In [Zhang et al 2022](https://doi.org/10.1038/s42003-022-03359-z  ) samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples). 
 
 
@@ -88,4 +94,4 @@ Heatmaps, PCA, and functional sets figures were created using Plotly.NETv4.0.0 (
 - Zhang, N., Mattoon, E.M., McHargue, W. et al. Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii. Commun Biol 5, 460 (2022). https://doi.org/10.1038/s42003-022-03359-z
 - featureCounts: Liao Y, Smyth GK, Shi W. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2014 Apr 1;30(7):923-30. 
 - Usadel B, Poree F, Nagel A, Lohse M, Czedik-Eysenberg A, Stitt M (2009) A guide to using MapMan to visualize and compare Omics data in plants: a case study in the crop species, Maize. Plant Cell Environment, 32: 1211-1229
-- May P, Christian JO, Kempa S, Walther D. ChlamyCyc: an integrative systems biology database and web-portal for Chlamydomonas reinhardtii. BMC Genomics. 2009 May 4;10:209. doi: 10.1186/1471-2164-10-209. PMID: 19409111; PMCID: PMC2688524.
\ No newline at end of file
+- May P, Christian JO, Kempa S, Walther D. ChlamyCyc: an integrative systems biology database and web-portal for Chlamydomonas reinhardtii. BMC Genomics. 2009 May 4;10:209. doi: 10.1186/1471-2164-10-209. PMID: 19409111; PMCID: PMC2688524.