Data added for S6_A1-A3

.gitattributes

@@ -13,3 +13,12 @@ assays/S4_A2_Purification[[:space:]]of[[:space:]]H-Pab1-MLLE[[:space:]]for[[:spa
 assays/S4_A3_Size[[:space:]]exclusion[[:space:]]chromotography[[:space:]]of[[:space:]]H-Pab1-MLLE/dataset/Size[[:space:]]exclusion[[:space:]]chromotography[[:space:]]of[[:space:]]H-Pab1-MLLE[[:space:]](P4794).xlsx filter=lfs diff=lfs merge=lfs -text
 assays/S4_A5_X-ray[[:space:]]structure[[:space:]]analysis[[:space:]]of[[:space:]]H-Pab1-MLLE/dataset/Pab1_MLLE[[:space:]]-[[:space:]]Upa1_PAM2.mtz filter=lfs diff=lfs merge=lfs -text
 assays/S5_A2_Protein[[:space:]]structure[[:space:]]prediction[[:space:]]using[[:space:]]Alphafold2/dataset/UMAG_15064_Vps8_Alphafold[[:space:]]predicted[[:space:]]model.pdb filter=lfs diff=lfs merge=lfs -text
+assays/S6_A2_Protein[[:space:]]structure[[:space:]]prediction[[:space:]]of[[:space:]]MKRN1[[:space:]]using[[:space:]]Alphafold2/dataset/Human[[:space:]]and[[:space:]]U.maydis[[:space:]]Mkrn1_Alphafold2[[:space:]]predicted[[:space:]]model.pse filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/Pab1MLLE[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/Rrm4MLLE3[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/V_Pab1MLLE[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]U.maydis[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.S8B)/V_Rrm4MLLE3[[:space:]]Bait-Ladder,[[:space:]]4296,[[:space:]]4297,[[:space:]]4298,[[:space:]]0134,[[:space:]]132,[[:space:]]224_Super1.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/HsPab1_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super4.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/HsUbr5MLLE_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super1.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/V_HsPab1_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super4.tif filter=lfs diff=lfs merge=lfs -text
+assays/S6_A3_Western[[:space:]]blot[[:space:]]analysis[[:space:]]of[[:space:]]GST[[:space:]]pull-down[[:space:]]experiments[[:space:]]using[[:space:]]human[[:space:]]PAM2L[[:space:]]variants/dataset/Western[[:space:]]blot[[:space:]]analysis[[:space:]]using[[:space:]]human[[:space:]]G-MLLE[[:space:]]variants[[:space:]]vs[[:space:]]Mkrn1[[:space:]]PAM2L[[:space:]]variants[[:space:]](Fig.6D)/V_HsUbr5MLLE_bait,[[:space:]]Prey-0162,0123,0146,0133,0205,0124,0308_super1.tif filter=lfs diff=lfs merge=lfs -text

assays/S6_A1_Multiple sequence alignment of Mkrn1 orthologs/dataset/Mkrn1 orthologs for MSA v10.dnd

+(
+(
+(
+(
+(
+(
+H.sapiens:0.02794,
+B.taurus:0.02807)
+:0.01137,
+R.norvegicus:0.03546)
+:0.11745,
+D.rerio:0.16961)
+:0.33380,
+T.cyperi:0.29794)
+:0.03717,
+M.antarcticus:0.29911)
+:0.05780,
+(
+(
+U.maydis:0.21955,
+K.brasiliensis:0.22529)
+:0.01231,
+(
+(
+P.hubeiensis:0.18465,
+S.reilianum:0.15633)
+:0.01693,
+U.hordei:0.19910)
+:0.02334)
+:0.00255,
+M.pennsylvanicum:0.22380);

assays/S6_A1_Multiple sequence alignment of Mkrn1 orthologs/dataset/Mkrn1 orthologs for MSA v10.msf

+PileUp
+
+
+
+   MSF:  529  Type: P    Check:   965   .. 
+
+ Name: U.maydis oo  Len:  529  Check:  3215  Weight:  9.0
+ Name: K.brasiliensis oo  Len:  529  Check:   294  Weight:  9.0
+ Name: P.hubeiensis oo  Len:  529  Check:  4753  Weight:  8.0
+ Name: S.reilianum oo  Len:  529  Check:  1461  Weight:  7.0
+ Name: U.hordei oo  Len:  529  Check:  2099  Weight:  8.0
+ Name: M.pennsylvanicum oo  Len:  529  Check:  1963  Weight:  9.0
+ Name: M.antarcticus oo  Len:  529  Check:  5194  Weight:  11.0
+ Name: T.cyperi oo  Len:  529  Check:  1892  Weight:  11.0
+ Name: H.sapiens oo  Len:  529  Check:  5734  Weight:  4.0
+ Name: B.taurus oo  Len:  529  Check:  5838  Weight:  4.0
+ Name: R.norvegicus oo  Len:  529  Check:  5553  Weight:  4.0
+ Name: D.rerio oo  Len:  529  Check:  2969  Weight:  8.0
+
+//
+
+
+
+U.maydis              .......... .......... .......... .......... .......... 
+K.brasiliensis        .......... .......... .......... .......... .......... 
+P.hubeiensis          .......... .......... .......... .......... .......... 
+S.reilianum           .......... .......... .......... .......... .......... 
+U.hordei              .......... .......... .......... .......... .......... 
+M.pennsylvanicum      .......... .......... .......... .......... .......... 
+M.antarcticus         .......... .......... .......... .......... .......... 
+T.cyperi              .......... .......... .......... .......... .......... 
+H.sapiens             MAEAATPGTT ATTSGAGAAA ATAAAASPTP IPTVTAPSLG AGGGGGGSDG 
+B.taurus              MAEAAAPGTT ATPSGAGAAA AAAAAASPTP IPAVTSLSPG AGGGGGGSDG 
+R.norvegicus          MAEAAAPGTT ATTSGAGAAA AAVAAASLTS IPTVAAPSPG AGGGGGGSDG 
+D.rerio               .ASTAAP... AVIGG..... .......... .......... .......... 
+
+
+U.maydis              .......... .......... .......... MHHETNSGTL PISSTPTEQN 
+K.brasiliensis        .......... .......... .......... MQAESSTGTL PGLSTQTKLD 
+P.hubeiensis          .......... .......... .......... MQSEASKSVL PCPPAFAKQD 
+S.reilianum           .......... .......... .......... MQAEASSSCL AAPSTRAKQD 
+U.hordei              .......... .......... .......... MQAEAGPSRS SGPASKAQQD 
+M.pennsylvanicum      .......... .......... .......... MQAEAGPSKL ADPSTQPAKH 
+M.antarcticus         .......... .......... .......... MQETAPSTSG AAASKTAKPS 
+T.cyperi              .......... .......... .......... .......... .......... 
+H.sapiens             SGGG...... .......WTK QVTCRYFMHG VCKEGDNCRY SHDLSDSPYS 
+B.taurus              GGGGGGGGGG GGGGGGGWTK QVTCRYFMHG VCKEGDNCRY SHDLSDSPYG 
+R.norvegicus          SGGG...... .......WTK QVTCRYFMHG VCKEGDNCRY SHDLSDSPYG 
+D.rerio               .......... .......WTK HVTCRYFMHG LCKEGENCRY SHDLSSCKQT 
+
+
+U.maydis              TPCRYFYRSG RCRRGDKCRF SHSSTRKPSQ PC....LATE TPSVEVRLSA 
+K.brasiliensis        KPCRYYLRSG RCRRGAKCLF SHDETRRPVQ PVDAAPSATD AGPSSSRLSA 
+P.hubeiensis          TPCRYFYRSG KCERGTKCIF SHSDT.RKSG PSEASVP... ....AARLSA 
+S.reilianum           TLCRYFYRSG KCKRGDKCIF SHDST.RDR. .......... ....VQQLSA 
+U.hordei              TPCRYFYRSG RCKRGDKCLF SHQRQ.RKGE PSDSAPSSSL KQSRTTPLSA 
+M.pennsylvanicum      TPCRYFFRSG RCRRGDKCQF SHKET.RQTC QSSTAPSSSP TPSPDERLSA 
+M.antarcticus         TPCRYYSRSG KCRRGDKCLF SHEQA.RQSE QS.AIPSPSA PTTSSVRLSA 
+T.cyperi              ..CRYFYRSG KCTRGDKCQF SHAQK.RESG QP........ ...GVPKSSS 
+H.sapiens             VVCKYFQR.G YCIYGDRCRY EHSKPLKQEE ATATELTTKS SLAASSSLSS 
+B.taurus              VVCKYFQR.G YCIYGDRCRY EHSKPLKQEE ATATDLTAKS SLAASSSLSS 
+R.norvegicus          VVCKYFQR.G YCVYGDRCRY EHSKPLKQEE VTATDLSAKP SPAASSSLPS 
+D.rerio               MICKFFQK.G CCAFGDRCRY EHTKPSKQDE VPSSKPSMPL TAAPLAGTPE 
+
+
+U.maydis              .......... .......... .......... .QAVAFTP.. .......... 
+K.brasiliensis        .......... .......... .......... .QASAFEP.. .......... 
+P.hubeiensis          .......... .......... .......... .QAGEFIP.. .......... 
+S.reilianum           .......... .......... .......... .RAAEFTP.. .......... 
+U.hordei              .......... .......... .......... .QAPDFTP.. .......... 
+M.pennsylvanicum      .......... .......... .......... .HAPEYTP.. .......... 
+M.antarcticus         .......... .......... .......... .QAPEYTPS. .......... 
+T.cyperi              .......... .......... .......... .TESQASP.. .......... 
+H.sapiens             IVGPLVEMNT GEAESRNSNF ATVGAGSEDW VNAIEFVPGQ PYCGRTAPSC 
+B.taurus              .VGPTVDMNM GEAESRNSNF ATVGAGSEDW VNAIEFVPGQ PYCGRTAPSS 
+R.norvegicus          GVGSLAEMNP GEAESRNPNF PTVGAGSEDW VNAIEFVPGQ PYCGRTAPSC 
+D.rerio               PVSDGPGGTT GAQEKPQ... ...GSGAVDW VNAAEFVPGQ PYCGRADPVL 
+
+
+U.maydis              .......... ...GTSSRSN LSASVQAFQP VARP...GNN DNGALQGVAS 
+K.brasiliensis        .......... ...DSSSRST LSASATDFLP TAQN...DSD DDDAWQDIPS 
+P.hubeiensis          .......... ...GSTCRSD LSARAQAFHS AATS.SDDDD DQDAWQDIPA 
+S.reilianum           .......... ...GPASRSS LSASAQAFQP ATQTDSSDDD DEGAWQDVAP 
+U.hordei              .......... ...RSAPQSQ LSAGAEPFEP SSQG....SR DEDAWQDVDP 
+M.pennsylvanicum      .......... ...TSRTQSQ LSATAQVFEP SLQIG...GV DQHEWQDEVP 
+M.antarcticus         .......... .ANTSKAQPH ASKRDEGWVD IAAPESASSE TTEEWEDVAG 
+T.cyperi              .......... ...SEAGPSR LSALATDFVP .......... .SQSLQEISE 
+H.sapiens             TEAPLQGSVT KEESEKEQTA VETKKQLCPY AAVGECRYGE NCVYLHGDSC 
+B.taurus              AEAPLQGSVT KEDAEKEQSA VETKKQLCPY AAVGECRYGE NCAYLHGDAC 
+R.norvegicus          TEVPLQGSVT KEESEKEPTT VETEKQLCPY AAVGECRYGE NCVYLHGDSC 
+D.rerio               CEGP..GPLI EEEYEKEQAN KEMKKQLCPY AAVGECRYGL NCAYLHGDVC 
+
+
+U.maydis              ERQSPSASSS KPKPKPI... .........M KGVAASVGSP NTARCDICME 
+K.brasiliensis        E...PSAKAP RPRSN..... .........L KGVPASVTS. NTLPCSICME 
+P.hubeiensis          DPQ..SSSAP ISNPMP.... .........M KGVPASISSP PASHCDICME 
+S.reilianum           EPQ..PATSK KPAPAV.... .........M KGVPASVSEN .TQPCYICME 
+U.hordei              AP....ASPI KPRAVP.... .........M KGVPASVSEN .TEPCGICME 
+M.pennsylvanicum      EPQPSASASA TNKSKLV... .........T IGVPASISQN .VEPCSICME 
+M.antarcticus         SNSLPTETTA LSTVES.... .........A APIPQPETTA DPQTCSVCFE 
+T.cyperi              DRSRQEAQSS VTSRQA.... .......... ....LKQSSG DAEACGICME 
+H.sapiens             DMCGLQVLHP MDAAQRSQHI KSCIEAHEKD MELSFAVQRS KDMVCGICME 
+B.taurus              DMCGLQVLHP VDAAQRSQHI KSCIEAHEKD MELSFAVQRS KDMVCGICME 
+R.norvegicus          DMCGLQVLHP MDAAQRSQHI KSCIEAHEKD MELSFAVQRS KDMVCGICME 
+D.rerio               DMCGLQVLHP SDTSQRSQHI RACIEAHEKD MEISFAIQRS KDMMCGVCME 
+
+
+U.maydis              VP........ TVYAQHINCN HLFCCPCLKT WRKQ...... RSQAKSKDCP 
+K.brasiliensis        LP........ TVYAQHPNCD HHFCPPCLNR WRRQ...... HTQAKNKDCP 
+P.hubeiensis          IP........ AVYAQHSNCD HLFCPPCLST WRRQ...... HDQSKNKNCP 
+S.reilianum           IP........ TVYAQQLNCD HLFCPPCLQK WRRQ...... HGQAKNKNCP 
+U.hordei              IP........ EVYAHHPNCD HSFCPSCLRE WRRQ...... HAQAKNKNCP 
+M.pennsylvanicum      VP........ AVYAQHINCD HFFCPPCLRQ WRRQ...... HGLAKIKNCP 
+M.antarcticus         VL........ KVFAQHPNCD HYFCPTCMRE WRGQ...... GDQDNRKKCP 
+T.cyperi              VP........ VVFAQHTGCN HYFCVPCIQQ WRKQRSVRGE QRQSNSKACP 
+H.sapiens             VVYEKANPSE RRFGILSNCN HTYCLKCIRK WRSAK....Q FESKIIKSCP 
+B.taurus              VVYEKANPSE RRFGILSNCN HTYCLKCIRK WRSAK....Q FESKIIKSCP 
+R.norvegicus          VVYEKANPSE RRFGILSNCN HTYCLKCIRK WRSAK....Q FESKIIKSCP 
+D.rerio               VVFEKTNPSE RRFGILSNCC HCYCLKCIRK WRSAK....Q FESKIIKSCP 
+
+
+U.maydis              TCRTPSDFTF VTPQPFTD.G ARTLALQRFR QRAADTPCKN FTKSLGLSN. 
+K.brasiliensis        VCRTASRFTF VTPQPFTG.A ARTLALARFR ERAAQTPCKN FEKSL..AS. 
+P.hubeiensis          TCRTHSRYTF VTPQPFAS.S ARTLALQRFR QRAAGTPCKN FSKSLALSS. 
+S.reilianum           TCRTPSQFTF VTPEPFVG.G ARSLALQRFR ERAAQTPCKH FTKSLALSS. 
+U.hordei              TCRTSSKFTF VTPQPFTS.G ARILALQRFK ERAATTPCKI FTKSLALSN. 
+M.pennsylvanicum      ICRASSHYTF VTPQPFTA.A ARTLALQRFR ERVASTPCKN FTKSLQLSK. 
+M.antarcticus         LCRVESKYTF VTGQPFQG.S ARTLTVQRFR QRAAATPCKA FTRSLSLSR. 
+T.cyperi              TCRSRSNFTF VTAEPVAN.Q QRLAAIERHR KRLKQIPCRH FTKSLGLHRS 
+H.sapiens             ECRITSNFVI PSEYWVEEKE EKQKLILKYK EAMSNKACRY FDEGRGSCP. 
+B.taurus              ECRITSNFVI PSEYWVEEKE EKQKLIQKYK EAMSNKACRY FDEGRGSCP. 
+R.norvegicus          ECRITSNFVI PSEYWVEEKE EKQKLIQKYK EAMSNKACRY FDEGRGSCP. 
+D.rerio               ECRITSNLVI PSEYWVEDKE EKQQLIQKYK DGMGTKPCRY FDEGRGTCP. 
+
+
+U.maydis              .......... .......... ...KRLTKPF CMFGDDCLYK HEMDGKEYKF 
+K.brasiliensis        .......... .......... ...RKGKEPF CMFGDECMYK HEVKGKEFKF 
+P.hubeiensis          .......... .......... ...KRSTHPF CIFGDDCMYK HEIDGKEYKF 
+S.reilianum           .......... .......... ...KRMTKPF CIFGDDCLYQ HHIDSQPHKF 
+U.hordei              .......... .......... ...KR.TKPF CVFGDDCLYQ HHINGQPYKF 
+M.pennsylvanicum      .......... .......... ...KR.MKPF CIYGDDCVYR HEIDTKPYKF 
+M.antarcticus         .......... .......... ...NR.NKPF CAFADDCLFQ HRIDGKSYTF 
+T.cyperi              LDSRSDTPAP AAAADPNADF SKGKKKATPY CVFGDDCLFQ HSIDGKPYLF 
+H.sapiens             .......... .......... ......FGGN CFYKHAYPDG RREEPQ..RQ 
+B.taurus              .......... .......... ......FGGN CFYKHAYPDG RREEPQ..RQ 
+R.norvegicus          .......... .......... ......FGGN CFYKHAYPDG RREEPQ..RQ 
+D.rerio               .......... .......... ......FGAN CFYKHAFPDG RLEEPQPQRR 
+
+
+U.maydis              EHGKYKIQES KKGTRRIVG. ..LGARAA.. .......... .......... 
+K.brasiliensis        GSGRVRINRG AKGRKRVART .TTGSRRSIG AEFFARIRDM DERVRIFRSS 
+P.hubeiensis          GQGKFRIQRS NKRLRRIVGL RGRGSERMAN VEFFTRIRDM DERVRMFLTT 
+S.reilianum           GTGRYRIQRG KRGTRRIVGL RG.QAERVIS TDFFARIQDM DERVRMFLST 
+U.hordei              GVGRYRIAHY RKGTKRLVRP SA.RTERTIG AEFFDRIRDM DERVRMFLAT 
+M.pennsylvanicum      GEGKHKINNG RKGIKRIVRP .VRGSRSSFA TEFHTTIQGM DARLRMFLST 
+M.antarcticus         GYGRLVVRKN RKRTRRLVRP .VARSESSMR PEFLQRVRDI DQRILLLLAA 
+T.cyperi              NAG.FKDMKA RR........ .......... .......... .......... 
+H.sapiens             KVGTSSRYRA QRRNHFWELI EERENSNPFD NDEEEVVTFE LGEMLLMLLA 
+B.taurus              KVGTSSRCRA QRRNHFWELI EERENGNPFD .DEEEVVTFE LGEMLLMLLA 
+R.norvegicus          KVGTSSRYRA QRRSHFWELI EEREN.NPFD NDEEEVVTFE LGEMLLMLLA 
+D.rerio               QNGSNGRNRN TRRTHLWDLL DERENSDSFD NEDEEMVRFE LSEMLLMLLA 
+
+
+U.maydis              .......... .......... .........
+K.brasiliensis        RSLTSGMGSQ LTLLRG.... .........
+P.hubeiensis          RSILSARSQI SAAS...... .........
+S.reilianum           RVILNARSQA SAA....... .........
+U.hordei              RVLVNARVAQ STGETHLE.. .........
+M.pennsylvanicum      RVLLSTRGNT TSHSSNPFYL .........
+M.antarcticus         RQAA...... .......... .........
+T.cyperi              .......... .......... .........
+H.sapiens             AGGDDELTDS EDEWDLFHDE LEDFYDLDL
+B.taurus              AGGDDELTDS EDEWDLFHDE LEDFYDLDL
+R.norvegicus          AGGDDELTDS EDEWDLFHDE LEDFYDLEL
+D.rerio               AGTDDDVTDS EDEWDLFHEE LDDYYELYL
+

assays/S6_A1_Multiple sequence alignment of Mkrn1 orthologs/dataset/Mkrn1 orthologs for MSA v10.txt

+>H.sapiens sp|Q9UHC7|MKRN1_HUMAN E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3 482AA
+MAEAATPGTTATTSGAGAAAATAAAASPTPIPTVTAPSLGAGGGGGGSDGSGGGWTKQVT
+CRYFMHGVCKEGDNCRYSHDLSDSPYSVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATAT
+ELTTKSSLAASSSLSSIVGPLVEMNTGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCG
+RTAPSCTEAPLQGSVTKEESEKEQTAVETKKQLCPYAAVGECRYGENCVYLHGDSCDMCG
+LQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMVCGICMEVVYEKANPSERRFG
+ILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEEKEEKQKLILK
+YKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRNHFW
+ELIEERENSNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLDL
+>R.norvegicus   NP_001385662.1 E3 ubiquitin-protein ligase makorin-1 isoform 1 [Rattus norvegicus]
+MAEAAAPGTTATTSGAGAAAAAVAAASLTSIPTVAAPSPGAGGGGGGSDGSGGGWTKQVTCRYFMHGVCK
+EGDNCRYSHDLSDSPYGVVCKYFQRGYCVYGDRCRYEHSKPLKQEEVTATDLSAKPSPAASSSLPSGVGS
+LAEMNPGEAESRNPNFPTVGAGSEDWVNAIEFVPGQPYCGRTAPSCTEVPLQGSVTKEESEKEPTTVETE
+KQLCPYAAVGECRYGENCVYLHGDSCDMCGLQVLHPMDAAQRSQHIKSCIEAHEKDMELSFAVQRSKDMV
+CGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEE
+KEEKQKLIQKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTSSRYRAQRRSHFW
+ELIEERENNPFDNDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYDLEL
+>B.taurus   XP_024846420.1 E3 ubiquitin-protein ligase makorin-1 isoform X1 [Bos taurus]
+MAEAAAPGTTATPSGAGAAAAAAAAASPTPIPAVTSLSPGAGGGGGGSDGGGGGGGGGGGGGGGGGGWTK
+QVTCRYFMHGVCKEGDNCRYSHDLSDSPYGVVCKYFQRGYCIYGDRCRYEHSKPLKQEEATATDLTAKSS
+LAASSSLSSVGPTVDMNMGEAESRNSNFATVGAGSEDWVNAIEFVPGQPYCGRTAPSSAEAPLQGSVTKE
+DAEKEQSAVETKKQLCPYAAVGECRYGENCAYLHGDACDMCGLQVLHPVDAAQRSQHIKSCIEAHEKDME
+LSFAVQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPECRITS
+NFVIPSEYWVEEKEEKQKLIQKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAYPDGRREEPQRQKVGTS
+SRCRAQRRNHFWELIEERENGNPFDDEEEVVTFELGEMLLMLLAAGGDDELTDSEDEWDLFHDELEDFYD
+LDL
+>D.rerio  AAG27598.2 Makorin RING zinc-finger protein 1, partial [Danio rerio]
+ASTAAPAVIGGWTKHVTCRYFMHGLCKEGENCRYSHDLSSCKQTMICKFFQKGCCAFGDRCRYEHTKPSK
+QDEVPSSKPSMPLTAAPLAGTPEPVSDGPGGTTGAQEKPQGSGAVDWVNAAEFVPGQPYCGRADPVLCEG
+PGPLIEEEYEKEQANKEMKKQLCPYAAVGECRYGLNCAYLHGDVCDMCGLQVLHPSDTSQRSQHIRACIE
+AHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSERRFGILSNCCHCYCLKCIRKWRSAKQFESKIIKSC
+PECRITSNLVIPSEYWVEDKEEKQQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDGRLEEPQ
+PQRRQNGSNGRNRNTRRTHLWDLLDERENSDSFDNEDEEMVRFELSEMLLMLLAAGTDDDVTDSEDEWDL
+FHEELDDYYELYL
+>U.maydis XP_011387489.1 uncharacterized protein UMAG_12122 [Ustilago maydis 521]
+MHHETNSGTLPISSTPTEQNTPCRYFYRSGRCRRGDKCRFSHSSTRKPSQPCLATETPSVEVRLSAQAVAFTPGTSSRSN
+LSASVQAFQPVARPGNNDNGALQGVASERQSPSASSSKPKPKPIMKGVAASVGSPNTARCDICMEVPTVYAQHINCNHLF
+CCPCLKTWRKQRSQAKSKDCPTCRTPSDFTFVTPQPFTDGARTLALQRFRQRAADTPCKNFTKSLGLSNKRLTKPFCMFG
+DDCLYKHEMDGKEYKFEHGKYKIQESKKGTRRIVGLGARAA
+>P.hubeiensis XP_012190908.1 hypothetical protein PHSY_004906 [Pseudozyma hubeiensis SY62]
+MQSEASKSVLPCPPAFAKQDTPCRYFYRSGKCERGTKCIFSHSDTRKSGPSEASVPAARLSAQAGEFIPGSTCRSDLSAR
+AQAFHSAATSSDDDDDQDAWQDIPADPQSSSAPISNPMPMKGVPASISSPPASHCDICMEIPAVYAQHSNCDHLFCPPCL
+STWRRQHDQSKNKNCPTCRTHSRYTFVTPQPFASSARTLALQRFRQRAAGTPCKNFSKSLALSSKRSTHPFCIFGDDCMY
+KHEIDGKEYKFGQGKFRIQRSNKRLRRIVGLRGRGSERMANVEFFTRIRDMDERVRMFLTTRSILSARSQISAAS
+>S.reilianum SJX61039.1 related to makorin ring zinc finger protein [Sporisorium reilianum f. sp. reilianum]
+MQAEASSSCLAAPSTRAKQDTLCRYFYRSGKCKRGDKCIFSHDSTRDRVQQLSARAAEFTPGPASRSSLSASAQAFQPAT
+QTDSSDDDDEGAWQDVAPEPQPATSKKPAPAVMKGVPASVSENTQPCYICMEIPTVYAQQLNCDHLFCPPCLQKWRRQHG
+QAKNKNCPTCRTPSQFTFVTPEPFVGGARSLALQRFRERAAQTPCKHFTKSLALSSKRMTKPFCIFGDDCLYQHHIDSQP
+HKFGTGRYRIQRGKRGTRRIVGLRGQAERVISTDFFARIQDMDERVRMFLSTRVILNARSQASAA
+>M.pennsylvanicum CDI51899.1 related to makorin ring zinc finger protein [Melanopsichium pennsylvanicum 4]
+MQAEAGPSKLADPSTQPAKHTPCRYFFRSGRCRRGDKCQFSHKETRQTCQSSTAPSSSPTPSPDERLSAHAPEYTPTSRT
+QSQLSATAQVFEPSLQIGGVDQHEWQDEVPEPQPSASASATNKSKLVTIGVPASISQNVEPCSICMEVPAVYAQHINCDH
+FFCPPCLRQWRRQHGLAKIKNCPICRASSHYTFVTPQPFTAAARTLALQRFRERVASTPCKNFTKSLQLSKKRMKPFCIY
+GDDCVYRHEIDTKPYKFGEGKHKINNGRKGIKRIVRPVRGSRSSFATEFHTTIQGMDARLRMFLSTRVLLSTRGNTTSHS
+SNPFYL
+>U.hordei    XP_041415531.1 uncharacterized protein UHO2_02160 [Ustilago hordei]
+MQAEAGPSRSSGPASKAQQDTPCRYFYRSGRCKRGDKCLFSHQRQRKGEPSDSAPSSSLKQSRTTPLSAQAPDFTPRSAP
+QSQLSAGAEPFEPSSQGSRDEDAWQDVDPAPASPIKPRAVPMKGVPASVSENTEPCGICMEIPEVYAHHPNCDHSFCPSC
+LREWRRQHAQAKNKNCPTCRTSSKFTFVTPQPFTSGARILALQRFKERAATTPCKIFTKSLALSNKRTKPFCVFGDDCLY
+QHHINGQPYKFGVGRYRIAHYRKGTKRLVRPSARTERTIGAEFFDRIRDMDERVRMFLATRVLVNARVAQSTGETHLE
+>K.brasiliensis KAF6767369.1 Zinc finger, CCCH-type [Kalmanozyma brasiliensis GHG001]
+MQAESSTGTLPGLSTQTKLDKPCRYYLRSGRCRRGAKCLFSHDETRRPVQPVDAAPSATDAGPSSSRLSAQASAFEPDSS
+SRSTLSASATDFLPTAQNDSDDDDAWQDIPSEPSAKAPRPRSNLKGVPASVTSNTLPCSICMELPTVYAQHPNCDHHFCP
+PCLNRWRRQHTQAKNKDCPVCRTASRFTFVTPQPFTGAARTLALARFRERAAQTPCKNFEKSLASRKGKEPFCMFGDECM
+YKHEVKGKEFKFGSGRVRINRGAKGRKRVARTTTGSRRSIGAEFFARIRDMDERVRIFRSSRSLTSGMGSQLTLLRG
+>M.antarcticus GAC72811.1 predicted E3 ubiquitin ligase [Moesziomyces antarcticus T-34]
+MQETAPSTSGAAASKTAKPSTPCRYYSRSGKCRRGDKCLFSHEQARQSEQSAIPSPSAPTTSSVRLSAQAPEYTPSANTS
+KAQPHASKRDEGWVDIAAPESASSETTEEWEDVAGSNSLPTETTALSTVESAAPIPQPETTADPQTCSVCFEVLKVFAQH
+PNCDHYFCPTCMREWRGQGDQDNRKKCPLCRVESKYTFVTGQPFQGSARTLTVQRFRQRAAATPCKAFTRSLSLSRNRNK
+PFCAFADDCLFQHRIDGKSYTFGYGRLVVRKNRKRTRRLVRPVARSESSMRPEFLQRVRDIDQRILLLLAARQAA
+>T.cyperi PWZ01012.1 hypothetical protein BCV70DRAFT_143119, partial [Testicularia cyperi]
+CRYFYRSGKCTRGDKCQFSHAQKRESGQPGVPKSSSTESQASPSEAGPSRLSALATDFVPSQSLQEISEDRSRQEAQSSV
+TSRQALKQSSGDAEACGICMEVPVVFAQHTGCNHYFCVPCIQQWRKQRSVRGEQRQSNSKACPTCRSRSNFTFVTAEPVA
+NQQRLAAIERHRKRLKQIPCRHFTKSLGLHRSLDSRSDTPAPAAAADPNADFSKGKKKATPYCVFGDDCLFQHSIDGKPY
+LFNAGFKDMKARR