add study1_tag integration

studies/1_TagIntegration/README.md

+Every possible combination was constructed (N-terminal, C-terminal, His, Strep, long linker (4x), short linker (2x)) in every possible combination in pK19-plasmids. However, only two promising were integrated on a first trial (N-Strep-4linker and C-His-2linker), leading to promising strain.
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studies/1_TagIntegration/protocols/Tag integration.md

+Plasmids were constructed by enzymatically assembling the generated DNA fragments into a cut vector backbone using Gibson assembly (Gibson et al., 2009). Sequencing of the final plasmid was performed by Eurofins Genomics (Ebersberg, Germany).
+For the integration of the His-Tag and a linker (GGGS2) at the C- or N-terminus of DtxR in the genome of C. glutamicum, the suicide vector pK19-mobsacB was used (Schäfer et al., 1994). Electrocompetent C. glutamicum cells were transformed with the isolated plasmid by electroporation (van der Rest et al., 1999). Then, the first and second recombination events were performed and verified as described in previous studies (Niebisch and Bott, 2001). The respective deletion was reviewed by amplification and sequencing (Eurofins Genomics, Ebersberg, Germany).