Merge branch 'hannah' into 'main'

merge Hannah

See merge request !6

assays/RNASeq_drought/README.md

+# Drought stress analysis in *Ribes nigrum*
+In a separate expreriment, the influence of drought stress on the gene expression was investigated. For this, RNA was extracted from roots and leaves. DNA was digested and RNA quality verified. RNA was reverse transcribed and PCR-amplified, using primers suitable for Oxford Nanopore (ONT) sequencing. Fragments were sequenced in-house on PromethION.
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assays/Ribes_nigrum_genome_sequencing/README.md

+# Genome sequencing
+For generating a *Ribes nigrum* genome assembly, the cultivar Rosenthals Langtraubige was used. DNA was extracted and filtered for long fragments above 40 kbp in order to perform Oxformd Nanopore (ONT) sequencing and PacBio sequencing. 
+
+Briefly, DNA was extracted, short fragments filtered out, DNA purity confirmed, and the libraries were prepared using the standard ONT protocol for the SQK-LSK112 kit. After DNA fragment repair and 3'-adenylation sequencing adapters were ligated to the DNA fragments, before a last purification step with AMPure XP beads. ONT sequencing was performed in-house, PacBio sequencing was performed by Genohub (Brigham Young University, Provo, UT, USA).
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