For generating a *Ribes nigrum* genome assembly, the cultivar Rosenthals Langtraubige was used. DNA was extracted and filtered for long fragments above 40 kbp in order to perform Oxford Nanopore (ONT) sequencing and PacBio sequencing.
For generating a *Ribes nigrum* genome, the cultivar Rosenthals Langtraubige was used. DNA was extracted and filtered for long fragments above 40 kbp in order to perform Oxford Nanopore (ONT) sequencing, PacBio sequencing and short read sequencing (Illumina).
Briefly, DNA was extracted, short fragments filtered out, DNA purity confirmed, and the libraries were prepared using the standard ONT protocol for the SQK-LSK112 kit. After DNA fragment repair and 3'-adenylation sequencing adapters were ligated to the DNA fragments, before a last purification step with AMPure XP beads. ONT sequencing was performed in-house, PacBio sequencing was performed by Genohub (Brigham Young University, Provo, UT, USA).
Briefly, DNA was extracted, short fragments filtered out, DNA purity confirmed, and the libraries were prepared using the standard ONT protocol for the SQK-LSK112 kit. After DNA fragment repair and 3'-adenylation sequencing adapters were ligated to the DNA fragments, before a last purification step with AMPure XP beads. ONT sequencing was performed in-house, PacBio sequencing was performed by Genohub (Brigham Young University, Provo, UT, USA). Short read sequencing was performed by Genewiz.
## Experimental workflow
## Experimental workflow
## Related repositories and files
The different steps performed for the R. nigrum genome were the following. The respective metadata and data can be found in the different folders:
[**Sequencing**](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)**=> You are here!**
