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Dominik Brilhaus
Facultative CAM in Talinum
Commits
71d631c6
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71d631c6
authored
3 years ago
by
Dominik Brilhaus
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**/dataset/** filter=lfs diff=lfs merge=lfs -text
/runs/run1/01_kallisto_index filter=lfs diff=lfs merge=lfs -text
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README.md
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# ARC mininmal Example RNASeq
This is a minimal Example ARC packaging an mRNA-Seq dataset with metadata and computations.
This is a minimal Example ARC packaging an mRNA-Seq dataset with metadata and computations.
## Data origin
-
Partly published under: https://doi.org/10.1104/pp.15.01076
-
see
[
./isa.investigation.xlsx
](
./isa.investigation.xlsx
)
for details.
-
Partly published under:
<https://doi.org/10.1104/pp.15.01076>
-
see
[
./isa.investigation.xlsx
](
./isa.investigation.xlsx
)
for details.
## Additional payload
> The following folders are not part of the ARC
> for details, see: [ARC specs:Additional paylod](https://github.com/nfdi4plants/ARC-specification/blob/main/ARC%20specification.md#additional-payload)
...
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@@ -14,34 +16,33 @@ Directory | Purpose
---- | ----
[
_GEO_submission
](
./_GEO_submission
)
| Example metadata files as required for submission to GEO
## Notes and ToDos
### Experimental metadata in isa.assay.xlsx
-
split GEO SWATE templates into four sheets
-
1SPL01_plants
-
2EXT01_RNA
-
3ASY01_RNASeq
-
4COM01_RNASeq
### Adding large raw data via git lfs
-
split GEO SWATE templates into four sheets
-
1SPL01_plants
-
2EXT01_RNA
-
3ASY01_RNASeq
-
4COM01_RNASeq
1.
Before addind the files to the ARC, track them via
`git lfs`
```
git lfs track "*.fastq.gz"
git lfs track "01_kallisto_index"
### Adding large raw data via git lfs
``
`
1.
Before addind the files to the ARC, track them via
`git lfs
`
2.
Move / add the large data files to the respective folders
```bash
git lfs track "*.fastq.gz"
git lfs track "01_kallisto_index"
```
3
.
Add them via
`git add`
1
.
Move / add the large data files to the respective folders
```
git add assays/Talinum_RNASeq_minimal/dataset/
git add runs/run1/01_kallisto_index
1.
Add them via
`git add`
```
```bash
git add assays/Talinum_RNASeq_minimal/dataset/
git add runs/run1/01_kallisto_index
```
4
.
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.
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ARC_root
=
"~/03_DataPLANT_gitlab/samplearc_rnaseq/"
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workflows/06_plot_shinyApp.Rmd
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@@ -14,7 +14,7 @@ knitr::opts_chunk$set(echo = TRUE)
# Load data
ARC_root="~/
Hackathon_ARCex
ample_rnaseq/"
ARC_root="~/
03_DataPLANT_gitlab/s
ample
arc
_rnaseq/"
load(file = paste0(ARC_root, 'runs/05_shinyPrep.RData'))
```
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