create plant resistance assay and add protocol

assays/AssessmentOfPlantResistanceToPseudomonasSyringae/protocols/AssessmentOfPlantResistanceToPseudomonasSyringaeProtocol.md

+## Assessment of plant resistance to *Pseudomonas syringae*
+
+To assess bacterial growth in naive 5-week-old plants, overnight log phase cultures of *Pseudomonas syringae* pathovar *maculicola* were washed three times with 10 mM MgCl2 and diluted to a final optical density at 600nm (OD600)=0.001 before infiltrating the resulting bacterial suspensions from the abaxial side into three fully grown leaves using a 1 ml syringe without a needle. The infiltration was performed between 10.00 h and 11.00 h, as previously described (DOI: 10.1016/j.cell.2018.02.049). Approximately 60 h later, the bacterial growth was quantified by measuring the bacterial bioluminescence in leaf discs (10 mm in diameter) of infiltrated leaves (one disc per leaf, three discs per plant) using a Sirius FB12 luminometer (Berthold Detection Systems). For each independent experiment, at least 18 replicate leaves from 6–7 plants per genotype were measured before performing a statistical analysis of the resulting values.
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