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Commit 5221b2cf authored by Viktoria Petrova's avatar Viktoria Petrova
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add qRT-PCR assay

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# A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley
## Original publication
Wanke, A., van Boerdonk, S., Mahdi, L. K., Wawra, S., Neidert, M., Chandrasekar, B., Saake, P., Saur, I. M. L., Derbyshire, P., Holton, N., Menke, F. L. H., Brands, M., Pauly, M., Acosta, I. F., Zipfel, C., & Zuccaro, A. (2023). A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley. Current biology : CB, 33(23), 5071–5084.e7. https://doi.org/10.1016/j.cub.2023.10.048
## Copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
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<img src=dataset\grp2_lrg.jpg width=60%>
### Figure 2 caption
**Figure 2.** Mutation of barley GBP1 GBP2 decreases colonization by the fungal pathogens B. sorokiniana and B. hordei
To test the impact of GBP mutation on the compatibility of pathogenic fungi, barley roots and shoots were inoculated with B. sorokiniana (left graph) and B. hordei (right graph), respectively. Barley control and gbp mutant lines were inoculated with B. sorokiniana and grown in jars under axenic conditions for 7 days (Figure S3C). To assess the degree of root colonization, the expression of B. sorokiniana housekeeping gene BsTEF was quantified by RT-PCR and normalized to the barley housekeeping gene ubiquitin HvUBI. Barley leaves colonized by B. hordei were analyzed for penetration success using bright-field microscopy (Figure S3D). Boxplot represents quantification of B. hordei penetration success at 48 h. Boxplot elements in this figure: center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. Data points from independent experiments are indicated by data point shape. Different letters represent statistically significant differences based on one-way ANOVA and Tukey’s post hoc test (significance threshold: p ≤ 0.05).
### Figure 2 source
https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#fx1
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assays/qRT-PCR/dataset/gr2_lrg.jpg

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