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Commit 8928327e authored by Viktoria Petrova's avatar Viktoria Petrova
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add study FungalMaterialGrowthConditionsAndBarleyColonizationAssays

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## Serendipita indica, Serendipita vermifera and Bipolaris sorokiniana
The growth conditions for barley, *S. indica* (DSM11827, Si), *S. vermifera* (MAFF305830, Sv) and *B. sorokiniana* (ND90Pr, Bs) and the preparation of fungal suspensions for plant inoculation have been described previously.41,75 Four-day-old barley seedlings were transferred to sterile jars on 1/10 PNM (pH 5.7) and inoculated with 3 mL of either sterile water as control, *Si* chlamydospores (500,000 spores·mL-1), *Sv* mycelium (1 g per 50 mL), or *Bs* conidia (5,000 spores·mL-1). Plants were grown on a day/night cycle of 16/8 h at 22/18 °C and 60% humidity under a light intensity of 108 μmol·m−2·s−1. Plant roots were harvested six days post inoculation (dpi), washed thoroughly to remove extraradical fungal hyphae, and frozen in liquid nitrogen. Four barley plants were pooled per biological replicate. To quantify fungal colonization and *PR10* gene expression, RNA extraction, cDNA generation and RT-PCR were performed as previously described.41
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