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Commit 93bb7745 authored by Viktoria Petrova's avatar Viktoria Petrova
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add supplementary figures captions in TLC assay

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...@@ -14,4 +14,36 @@ https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=h ...@@ -14,4 +14,36 @@ https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=h
**Figure 4 A).** The digestion products resulting from the enzymatic breakdown of laminarin by GBP1 over time were analyzed using thin-layer chromatography (TLC). Untreated (UT) and GBP1E500A-treated laminarin (20 h) served as controls. Digestion was performed at 25°C. **Figure 4 A).** The digestion products resulting from the enzymatic breakdown of laminarin by GBP1 over time were analyzed using thin-layer chromatography (TLC). Untreated (UT) and GBP1E500A-treated laminarin (20 h) served as controls. Digestion was performed at 25°C.
### Figure 4 A) source ### Figure 4 A) source
https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065 https://www.cell.com/current-biology/fulltext/S0960-9822(23)01449-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982223014495%3Fshowall%3Dtrue#secsectitle0065
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<img src=dataset\FigS4A.jpg width=60%>
### Supplementary Figure 4 A) caption
**Supplementary Figure 4 A).** Laminarin (4 mg·mL−1) was digested with GBP1 (70 nM) for 10 min at different temperatures. Sample without enzymes (UT) was mock-digested at 80 °C. Products from GBP1-catalyzed laminarin digestion assays in (A) and (B) were separated by thin layer
chromatography. Quantification of thin layer chromatographs in Figure 4C.
### Supplementary Figure 4 A) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
<img src=dataset\FigS4B.jpg width=60%>
### Supplementary Figure 4 B) caption
**Supplementary Figure 4 B).** Laminarin (4 mg·mL−1) was digested with purified GBP1 (70 nM) in different buffers
(10 mM, pH 5-9). Digestions were performed at 60 °C for 10 min. Products from GBP1-
catalyzed laminarin digestion assays in (A) and (B) were separated by thin layer
chromatography. Quantification of thin layer chromatographs in Figure 4C.
### Supplementary Figure 4 B) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
<img src=dataset\FigS5A.jpg width=60%>
### Supplementary Figure 5 A) caption
**Supplementary Figure 5 A).** Laminarin from E. bicyclis (2.4 mM) was digested with GBP1 (72 nM) for 1 h at 60 °C.
The digestion products were analyzed by thin layer chromatography.
### Supplementary Figure 5 A) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
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