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Commit fcc3ff59 authored by Viktoria Petrova's avatar Viktoria Petrova
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delete old study and add a new protocol

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## Hordeum vulgare
All experiments, including the generation of CRISPR/Cas9 knock-out lines, were performed with the spring barley (*H. vulgare L.*) cv. Golden Promise Fast, an introgression line carrying the Ppd-H1 allele that confers fast flowering.42 From here on, we use “control” to name this non-mutagenized cultivar that carries normal copies of *GBP1* and *GBP2*. For ROS burst assays, barley seeds were surface sterilized with 6% sodium hypochlorite for 1 h and then washed extensively (5 × 30 mL sterile water). Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands).82 Seedlings were cultured for four days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 °C/18 °C, light intensity of 108 μmol·m−2·s−1).
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## Nicotiana benthamiana
Seeds of *N. benthamiana* wild-type lines were sown on soil and grown for 3 weeks in the greenhouse under long-day conditions (day/night cycle of 16/8 h, 22–25 °C, light intensity of ∼140 μmol·m−2·s−1, maximal humidity of 60%).
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