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Commit 005c10c3 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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## Growth of *lwd1* mutant plants
- plants were grown until maturity
**Growth conditions:**
- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- 1.5 L pots (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
## Propagation of *lwd1* mutant plants
- plants were grown until full maturity
- seeds were harvested for each plant individually
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## Propagation of *lwd1* mutant plants
- plants were grown in LD conditions until maturity
- seeds were harvested for each plant individually
**Generations:**
- T0 plants: regenerated plants from cell culture (embryo transformation), produce T1 seeds
- T1 plants: genotyping for potential mutants, produce T2 seeds
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