Skip to content
Snippets Groups Projects
Commit 10bf9ee5 authored by Gesa Helmsorig's avatar Gesa Helmsorig
Browse files

added genotyping data and protocol

parent 9ae07ff9
No related branches found
No related tags found
No related merge requests found
No preview for this file type
**F1 plants of lwd/eam7 allelism test: genoypting **
- DNA was extracted from leaves of all plants using QIAGEN_DNeasy-Plant_mini protocol
- plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')
- the PCR fragments were Sanger sequenced using two different primers:
- LWD1_71us_F (test the beginning of the CDS)
- LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3') (test the end of the CDS)
- all mutations and the zygosity of the mutations were determined relative to the start codon of LWD1
- all F1 plants were genotyped
- only some parent plants were genotyped again
\ No newline at end of file
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment