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Update file diurnal_plant_material_growth_conditions.md

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**eam7 diurnal expression: plant material and growth conditions**
## *eam7* diurnal expression: plant material and growth conditions
**plant material*
**Plant material:**
species: Hordeum vulgare
genotypes:
Species: *Hordeum vulgare*
Genotypes:
- BW: Bowman, spring cultivar
- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
......@@ -11,33 +12,33 @@ genotypes:
- BW(Ppd-H1, eam7): cross of the two introgression lines
**growth condtions**
**Growth condtions:**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot 96T trays (HerkuPlast Kubern GmbH, pot volume 75 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**sampling**
**Sampling:**
- 2 weeks old plants
- youngest, fully elongated leaf: leaf 2
- sample every 2 hours over 24 hours:
ZT0 (lights are turned on)
ZT2
ZT4
ZT6
ZT8 (lights are turned off)
ZT10
ZT12
ZT14
ZT16
ZT18
ZT20
ZT22
- ZT0 (lights are turned on)
- ZT2
- ZT4
- ZT6
- ZT8 (lights are turned off)
- ZT10
- ZT12
- ZT14
- ZT16
- ZT18
- ZT20
- ZT22
**References**
**References:**
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
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