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Commit 738df60a authored by Gesa Helmsorig's avatar Gesa Helmsorig
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Update 2 files

- /assays/MSA_dissection/protocols/dissection_of_MSAs.txt
- /assays/MSA_dissection/protocols/dissection_of_MSAs.md
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## Dissection of main shoot apices (MSAs) of barley plants
**Growth:**
- per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD) - per genotype: choose approx. 4 random plants every 6-8 days (SD) or every 2-10 days (LD)
- start 5 days after emergence (DAE) - start 5 days after emergence (DAE)
**Dissection:**
- remove the leaves surrounding the main shoot apex (MSA) with a blade - remove the leaves surrounding the main shoot apex (MSA) with a blade
- image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera - image MSA with the stereo microscope Nikon SMZ18 with a Nikon DS-Fi2 camera
- score developmental stage according to Waddington et al. (1983) - score developmental stage according to Waddington et al. (1983)
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