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Commit 7a043b18 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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- /studies/phenotyping_eam7_plants/protocols/plant_material_growth_conditions.md
- /studies/phenotyping_eam7_plants/README.md
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**Phenotype *eam7* plants under SD and LD conditions** ## Phenotype *eam7* plants under SD and LD conditions
Grow plants under short-day (SD) and long-day (LD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry. Grow plants under short-day (SD) and long-day (LD) conditions. Score flowering time and phenotypic traits at flowering and at harvest when plants are fully dry.
**study overview** **Study overview:**
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD
-> assay: plant_phenotyping -> assay: plant_phenotyping
output: output:
SD plants SD plants
- flowering time (in DAE) - flowering time (in DAE)
- at flowering: plant height, leaf number, tiller number, flag leaf length/width - at flowering: plant height, leaf number, tiller number,
- at harvest (on main culm): spike length, floret and grain number, spike fertility flag leaf length/width
- at harvest (on main culm): spike length, floret and grain number,
spike fertility
LD plants LD plants
- flowering time (in DAE) - flowering time (in DAE)
- at flowering: plant height, leaf number, tiller number - at flowering: plant height, leaf number, tiller number
- at harvest (on main culm): spike length, floret and grain number, spike fertility - at harvest (on main culm): spike length, floret and grain number,
spike fertility
Results can be found in Figure 1A and B, Figure 2, and Supplemental Figure S2. Results can be found in Figure 1A and B, Figure 2, and Supplemental Figure S2.
**References** **References:**
Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434 Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
**Plant material and growth conditions** ## Plant material and growth conditions
**plant material** **Plant material:**
species: Hordeum vulgare Species: *Hordeum vulgare*
genotypes: Genotypes:
- BW: Bowman, spring cultivar - BW: Bowman, spring cultivar
- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011) - BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011) - BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
- BW(Ppd-H1, eam7): cross of the two introgression lines - BW(Ppd-H1, eam7): cross of the two introgression lines
**growth condtions** **Growth condtions:**
- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR) - long day: (LD) 16h light / 8h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3) - QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.) - Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
**References** **References:**
Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249 Druka A, Franckowiak J, Lundqvist U, Bonar N, Alexander J, Houston K, Radovic S, Shahinnia F, Vendramin V, Morgante M, et al. Genetic dissection of barley morphology and development. Plant Physiol. 2011:155(2):617–627. https://doi.org/10.1104/pp.110.166249
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