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CEPLAS
Helmsorig-2024-eam7
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9b149194
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9b149194
authored
6 months ago
by
Gesa Helmsorig
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assays/complementation_crosses/protocols/crossing_plants.md
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**c
rossing of plants
**
## C
rossing of plants
**Growth:**
-
grow parent plants in LD conditions
-
if parental plants have very different flowering times: sow the faster one with a bit of delay)
**Crossing:**
-
emasculate florets from mother plants before pollen are shed (about Waddington stage 8.5 - 9.0)
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pollinate florets with pollen from father plants, pollinate all florets from one spike with the same father: this is one cross
-
cover the spike with a paper bag, count later how many of the pollinated florets develop into seeds
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