Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence.
**Generating *lwd1* mutants**
Using CRISPR-Cas9 to generate *lwd1* mutants to confirm *LWD1* as the gene underlying the *eam7* locus. GP-fast embryos were transformed with vectors carrying sgRNAs that target the start or the end of the *LWD1* coding sequence. The resulting T0 plants were genotyped to screen for different induced mutations. Promising T1 plants were sown, and genotyped again. From this, three different homozygous mutants were selected for gene expression analysis and phenotyping: *lwd1-26*, *lwd1-390* and *lwd1-402*.
**study overview**
WT transformation plants (GP-fast)
-> assay: embryo transformation
output: T0 mutant plants (used in study "genotyping_lwd1_mutants"
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output: *T0 mutant plants*
-> assay: plant_genotyping
output: T0 mutant genotype info
-> assay: plant propagation
output: *T1 mutant plants*
-> assay: plant_genotyping
output: T1 mutant genotype info
-> assay: plant propagation
output: T2 mutant plants (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants)