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Commit d20c1929 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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studies/gene_expression_lwd1_mutants_diurnal/README.md
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**Test diurnal gene expression of circadian clock and flowering time genes in *lwd1* mutants and WT plants** ## Test diurnal gene expression of circadian clock and flowering time genes in *lwd1* mutants and WT plants
2-week old plants from the genotypes *lwd1-26*, *lwd1-390*, *lwd1-402*, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20. 2-week old plants from the genotypes *lwd1-26*, *lwd1-390*, *lwd1-402*, and GP-fast were sampled at Zeitgeber Time (ZT) 0, 4, 8, 12, 16, and 20.
Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested. Gene expression of CO1, ELF3, FT1, LUX1, LWD1, PHYB, PHYC, Ppd-H1, and PRR1 were tested.
**study overview** **Study overview:**
input: input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD. input: 2-weeks old lwd1-26, lwd1-390, lwd1-402 and GP-fast plants grown under SD.
-> assay: leaf_sampling -> assay: leaf_sampling
output: frozen leaf tissue (3 biol. replicates) output: frozen leaf tissue (3 biol. replicates)
-> assay: RNA_extraction -> assay: RNA_extraction
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