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Identification of nuclear pore proteins at plasmodesmata

Identification of nuclear pore proteins at plasmodesmata

Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.

Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.

Genome sequencing of blackcurrant (Ribes nigrum)

"QUINOA DIVERSITY- Quinoa Phenotypic and Genotypic Diversity for Yield and Composition"

"QUINOA DIVERSITY- Quinoa Phenotypic and Genotypic Diversity for Yield and Composition"

MAdLand Project - Höcker Lab

Co-action of COP1, SPA and cryptochrome in light signal transduction and photomorphogenesis of the moss Physcomitrium patens

The Arabidopsis COP1/SPA ubiquitin ligase suppresses photomorphogenesis in darkness. In the light, photoreceptors inactivate COP1/SPA to allow a light response. While SPA genes are specific to the green lineage, COP1 also exists in humans. This raises the question of when in evolution plant COP1 acquired the need for SPA accessory proteins. We addressed this question by generating Physcomitrium Ppcop1 mutants and comparing their visible and molecular phenotypes with those of Physcomitrium Ppspa mutants. The phenotype of Ppcop1 nonuple mutants resembles that of Ppspa mutants. Most importantly, both mutants produce green chloroplasts in complete darkness. They also exhibit dwarfed gametophores, disturbed branching of protonemata and absent gravitropism. RNA-sequencing analysis indicates that both mutants undergo weak constitutive light signaling in darkness. PpCOP1 and PpSPA proteins form a complex and they interact via their WD repeat domains with the VP motif of the cryptochrome CCE domain in a blue light-dependent manner. This resembles the interaction of Arabidopsis SPA proteins with Arabidopsis CRY1, and is different from that with Arabidopsis CRY2. Taken together, the data indicate that PpCOP1 and PpSPA act together to regulate growth and development of Physcomitrium. However, in contrast to their Arabidopsis orthologs, PpCOP1 and PpSPA proteins execute only partial suppression of light signaling in darkness. Hence, additional repressors may exist that contribute to the repression of a light response in dark-exposed Physcomitrium.

The Plant Journal 114: 159–175; https://doi.org/10.1111/tpj.16128

NGS course within Master module "Plant Genetics" at the University of Cologne

Arabidopsis molecular responses (transcript, metabolite) to single, double, and triple combinations of salt, osmotic, and heat stresses.

This ARC presents diverse data from MibiNet members to illustrate the application of MibiNet-specific SWATE templates and showcase examples of metadata annotation.

This ARC is based on the research of Mönchgesang et al. 2016, who performed a metabolite profiling of 19 Arabidopsis thaliana accessions. The natural variability of root metabolic patterns was analyzed between different accessions, with the result that plant-to-plant variability is greater than natural variation between accessions and non-biological variation between experimental batches.