added data

studies/gene_expression_eam7_plants_diurnal/protocols/development_plant_material_growth_conditions.md

+**eam7 developmental expression: plant material and growth conditions**
+
+**plant material*
+
+species: Hordeum vulgare
+genotypes:
+
+- BW:  Bowman, spring cultivar
+- BW(Ppd-H1): Ppd-H1 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(eam7): eam7 introgression line BW281 in BW background, Druka et al. (2011)
+- BW(Ppd-H1, eam7): cross of the two introgression lines
+
+
+**growth condtions**
+
+- short day (SD): 8h light / 16h dark, 20/16 °C, 60% humidity, ~ 250 PAR)
+- QuickPot E24/10 trays (HerkuPlast Kubern GmbH, pot volume 480 cm^3)
+- Einheitserde ED73 (Einheitserde Werkverband e.V.) with 7% sand and 4 g/L Osmocote Exact Hi.End 3-4M, 4th generation (ICL Group Ltd.)
+
+**sampling**
+
+- always sample youngest, fully elongated leaf
+- sample at ZT 9 (1h after lights turned off, as FT1 expression is highest)
+- sample genotypes at 19, 26, 33, 40, 47 and 59 DAE
\ No newline at end of file

studies/gene_expression_eam7_plants_diurnal/protocols/plant_material_growth_conditions.md → studies/gene_expression_eam7_plants_diurnal/protocols/diurnal_plant_material_growth_conditions.md

-**Plant material and growth conditions**
+**eam7 diurnal expression: plant material and growth conditions**
 
 **plant material*
 
@@ -20,7 +20,9 @@ genotypes:
 
 **sampling**
 
-sample every 2 hours over 24 hours: 
+- 2 weeks old plants
+- youngest, fully elongated leaf: leaf 
+- sample every 2 hours over 24 hours: 
 	ZT0 (lights are turned on)
 	ZT2
 	ZT4