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S1_A1_Protein structure prediction
S1_A2_Protein Purification for structural studies
S1_A3_Size exclusion chromatography of H-Rrm4-MLLE3
S1_A4_Western blot analysis of GST pull-down experiments with MLLE3 versions
S1_A5_Isothermal Titration Calorimetry_ITC_using Rrm4 MLLE3
S1_A6_X-ray structure of Rrm4-MLLE3
S1_A7_Multiple sequence alignment of Rrm4-MLLE3 orthologs of representative fungi from Basidiomycota
S2_A1_Structural analysis of MLLE3 of Rrm4
S2_A2_Western blot analysis of GST pull-down experiments with MLLE3 variants
S3_A1_Western blot analysis of GST pull-down experiments with GST-MLLE3 and PAM2L variants
S3_A2_Structural comparison of MLLE3 in U_maydis and R_irregularis
S3_A3_Western blot analyis of Rrm4-MLLE3 from U_maydis and R_irregularis
S4_A1_Hyphal growth of strains expressing Rrm4-Kat with MLLE3 variants in AB33 background
S4_A2_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-MLLE variants
S4_A3_Micrographs and Kymograph measurement of Rrm4-Kat_MLLE3 variants movement in hyphae
S4_A4_Quantification of processive Rrm4-Kat signals - velocity and distance travelled
S5_A1_Protein structure prediction
S5_A2_Purification of H-Pab1-MLLE for structural studies
S5_A3_Size exclusion chromotography of H-Pab1-MLLE
S5_A4_Isothermal Titration calorimetry-ITC with H-Pab1-MLLE
S5_A5_X-ray structure analysis of H-Pab1-MLLE
S5_A6_Western blot analysis of GST pull-down experiments using G-Pab1-MLLE and PAM2 variants
S5_A7_Multiple sequence alignment of Pab1-MLLE orthologs
S6_A1_Multiple Sequence alignment of vps8 and Taf7 orthologs
S6_A2_Protein structure prediction using Alphafold2
S6_A3_Western blot analysis of GST pull-down experiments using predicted PAM2L variants
S6_A4_Hyphal growth of strains expressing Rrm4-Kat and Vps8-Gfp variants in AB33 background
S6_A5_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-Kat and Vps8-Gfp variants
S6_A6_Micrographs and Kymograph measurement of Rrm4-Kat movement in Vps8-Gfp variant hypha
S6_A7_Quantification of processive Rrm4-Kat signals_velocity and distance travelled in vps8-gfp variant hypha
S6_A8_Quantification of Rrm4-Kat signal accumulated at the tip
S7_A1_Multiple sequence alignment of Mkrn1 orthologs
S7_A2_Protein structure prediction of MKRN1 using Alphafold2
S7_A3_Western blot analysis of GST pull-down experiments using human PAM2L variants
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