Protocols from manuscript added to all the assays

a471cbc3 · Senthil-Kumar Devan · c793d2fe · a471cbc3 · a471cbc3 · a471cbc3
Commit a471cbc3 authored 3 months ago by Senthil-Kumar Devan
--- a/assays/S1_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx
+++ b/assays/S1_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx
--- a/assays/S1_A2_Protein Purification for structural studies/protocols/Recombinant protein expression and purification.docx
+++ b/assays/S1_A2_Protein Purification for structural studies/protocols/Recombinant protein expression and purification.docx
--- a/assays/S1_A3_Size exclusion chromatography of H-Rrm4-MLLE3/protocols/Recombinant protein purification.docx
+++ b/assays/S1_A3_Size exclusion chromatography of H-Rrm4-MLLE3/protocols/Recombinant protein purification.docx
--- a/assays/S1_A4_Western blot analysis of GST pull-down experiments with MLLE3 versions/protocols/GST Pulldown and Western blotting.docx
+++ b/assays/S1_A4_Western blot analysis of GST pull-down experiments with MLLE3 versions/protocols/GST Pulldown and Western blotting.docx
--- a/assays/S1_A5_Isothermal Titration Calorimetry_ITC_using Rrm4 MLLE3/protocols/Isothermal titration calorimetry (ITC).docx
+++ b/assays/S1_A5_Isothermal Titration Calorimetry_ITC_using Rrm4 MLLE3/protocols/Isothermal titration calorimetry (ITC).docx
--- a/assays/S1_A6_X-ray structure of Rrm4-MLLE3/protocols/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx
+++ b/assays/S1_A6_X-ray structure of Rrm4-MLLE3/protocols/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx
--- a/assays/S1_A7_Multiple sequence alignment of Rrm4-MLLE3 orthologs of representative fungi from Basidiomycota/protocols/Multiple Sequence alignment.docx
+++ b/assays/S1_A7_Multiple sequence alignment of Rrm4-MLLE3 orthologs of representative fungi from Basidiomycota/protocols/Multiple Sequence alignment.docx
--- a/assays/S2_A1_Structural analysis of MLLE3 of Rrm4/protocols/Structural analysis.docx
+++ b/assays/S2_A1_Structural analysis of MLLE3 of Rrm4/protocols/Structural analysis.docx
--- a/assays/S2_A2_Western blot analysis of GST pull-down experiments with MLLE3 variants/protocols/GST Pulldown and Western blotting.docx
+++ b/assays/S2_A2_Western blot analysis of GST pull-down experiments with MLLE3 variants/protocols/GST Pulldown and Western blotting.docx
--- a/assays/S3_A1_Western blot analysis of GST pull-down experiments with GST-MLLE3 and PAM2L variants/protocols/GST Pulldown and Western blotting.docx
+++ b/assays/S3_A1_Western blot analysis of GST pull-down experiments with GST-MLLE3 and PAM2L variants/protocols/GST Pulldown and Western blotting.docx
--- a/assays/S3_A2_Structural comparison of MLLE3 in U_maydis and R_irregularis/protocols/Structural analysis.docx
+++ b/assays/S3_A2_Structural comparison of MLLE3 in U_maydis and R_irregularis/protocols/Structural analysis.docx
--- a/assays/S3_A3_Western blot analyis of Rrm4-MLLE3 from U_maydis and R_irregularis/protocols/GST Pulldown and Western blotting.docx
+++ b/assays/S3_A3_Western blot analyis of Rrm4-MLLE3 from U_maydis and R_irregularis/protocols/GST Pulldown and Western blotting.docx
--- a/assays/S4_A1_Hyphal growth of strains expressing Rrm4-Kat with MLLE3 variants in AB33 background/protocols/Haphal growth and Microscopy data analysis.docx
+++ b/assays/S4_A1_Hyphal growth of strains expressing Rrm4-Kat with MLLE3 variants in AB33 background/protocols/Haphal growth and Microscopy data analysis.docx
--- a/assays/S4_A2_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-MLLE variants/protocols/Microscopy data analysis.docx
+++ b/assays/S4_A2_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-MLLE variants/protocols/Microscopy data analysis.docx
--- a/assays/S4_A3_Micrographs and Kymograph measurement of Rrm4-Kat_MLLE3 variants movement in hyphae/protocols/Microscopy data analysis.docx
+++ b/assays/S4_A3_Micrographs and Kymograph measurement of Rrm4-Kat_MLLE3 variants movement in hyphae/protocols/Microscopy data analysis.docx
--- a/assays/S4_A4_Quantification of processive Rrm4-Kat signals - velocity and distance travelled/protocols/Microscopy data analysis.docx
+++ b/assays/S4_A4_Quantification of processive Rrm4-Kat signals - velocity and distance travelled/protocols/Microscopy data analysis.docx
--- a/assays/S5_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx
+++ b/assays/S5_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx
--- a/assays/S5_A2_Purification of H-Pab1-MLLE for structural studies/protocols/Recombinant protein expression and purification.docx
+++ b/assays/S5_A2_Purification of H-Pab1-MLLE for structural studies/protocols/Recombinant protein expression and purification.docx
--- a/assays/S5_A3_Size exclusion chromotography of H-Pab1-MLLE/protocols/Recombinant protein purification.docx
+++ b/assays/S5_A3_Size exclusion chromotography of H-Pab1-MLLE/protocols/Recombinant protein purification.docx
--- a/assays/S5_A4_Isothermal Titration calorimetry-ITC with H-Pab1-MLLE/protocols/Isothermal titration calorimetry (ITC).docx
+++ b/assays/S5_A4_Isothermal Titration calorimetry-ITC with H-Pab1-MLLE/protocols/Isothermal titration calorimetry (ITC).docx