Protocols from manuscript added to all the assays (a471cbc3) · Commits · SFB1535_MibiNet / 2024_Devan-Sai_et_al_PNAS

Commit a471cbc3 authored 4 months ago by Senthil-Kumar Devan

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Protocols from manuscript added to all the assays

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assays/S1_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx 0 additions, 0 deletions

...otocols/Structure prediction, modelling and analysis.docx
assays/S1_A2_Protein Purification for structural studies/protocols/Recombinant protein expression and purification.docx 0 additions, 0 deletions

...cols/Recombinant protein expression and purification.docx
assays/S1_A3_Size exclusion chromatography of H-Rrm4-MLLE3/protocols/Recombinant protein purification.docx 0 additions, 0 deletions

...rm4-MLLE3/protocols/Recombinant protein purification.docx
assays/S1_A4_Western blot analysis of GST pull-down experiments with MLLE3 versions/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions

...versions/protocols/GST Pulldown and Western blotting.docx
assays/S1_A5_Isothermal Titration Calorimetry_ITC_using Rrm4 MLLE3/protocols/Isothermal titration calorimetry (ITC).docx 0 additions, 0 deletions

...LE3/protocols/Isothermal titration calorimetry (ITC).docx
assays/S1_A6_X-ray structure of Rrm4-MLLE3/protocols/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx 0 additions, 0 deletions

...ls/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx
assays/S1_A7_Multiple sequence alignment of Rrm4-MLLE3 orthologs of representative fungi from Basidiomycota/protocols/Multiple Sequence alignment.docx 0 additions, 0 deletions

... Basidiomycota/protocols/Multiple Sequence alignment.docx
assays/S2_A1_Structural analysis of MLLE3 of Rrm4/protocols/Structural analysis.docx 0 additions, 0 deletions

...lysis of MLLE3 of Rrm4/protocols/Structural analysis.docx
assays/S2_A2_Western blot analysis of GST pull-down experiments with MLLE3 variants/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions

...variants/protocols/GST Pulldown and Western blotting.docx
assays/S3_A1_Western blot analysis of GST pull-down experiments with GST-MLLE3 and PAM2L variants/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions

...variants/protocols/GST Pulldown and Western blotting.docx
assays/S3_A2_Structural comparison of MLLE3 in U_maydis and R_irregularis/protocols/Structural analysis.docx 0 additions, 0 deletions

...ydis and R_irregularis/protocols/Structural analysis.docx
assays/S3_A3_Western blot analyis of Rrm4-MLLE3 from U_maydis and R_irregularis/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions

...egularis/protocols/GST Pulldown and Western blotting.docx
assays/S4_A1_Hyphal growth of strains expressing Rrm4-Kat with MLLE3 variants in AB33 background/protocols/Haphal growth and Microscopy data analysis.docx 0 additions, 0 deletions

...protocols/Haphal growth and Microscopy data analysis.docx
assays/S4_A2_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-MLLE variants/protocols/Microscopy data analysis.docx 0 additions, 0 deletions

...rm4-MLLE variants/protocols/Microscopy data analysis.docx
assays/S4_A3_Micrographs and Kymograph measurement of Rrm4-Kat_MLLE3 variants movement in hyphae/protocols/Microscopy data analysis.docx 0 additions, 0 deletions

...ovement in hyphae/protocols/Microscopy data analysis.docx
assays/S4_A4_Quantification of processive Rrm4-Kat signals - velocity and distance travelled/protocols/Microscopy data analysis.docx 0 additions, 0 deletions

...istance travelled/protocols/Microscopy data analysis.docx
assays/S5_A1_Protein structure prediction/protocols/Structure prediction, modelling and analysis.docx 0 additions, 0 deletions

...otocols/Structure prediction, modelling and analysis.docx
assays/S5_A2_Purification of H-Pab1-MLLE for structural studies/protocols/Recombinant protein expression and purification.docx 0 additions, 0 deletions

...cols/Recombinant protein expression and purification.docx
assays/S5_A3_Size exclusion chromotography of H-Pab1-MLLE/protocols/Recombinant protein purification.docx 0 additions, 0 deletions

...Pab1-MLLE/protocols/Recombinant protein purification.docx
assays/S5_A4_Isothermal Titration calorimetry-ITC with H-Pab1-MLLE/protocols/Isothermal titration calorimetry (ITC).docx 0 additions, 0 deletions

...LLE/protocols/Isothermal titration calorimetry (ITC).docx

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