add assay 2_1

.gitattributes

 assays/1-1_GrowthMonitoring-taggedVariants/dataset/20210218_BiolectorAK59_raw.csv filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Background/His-Background.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Background/Strep-Background.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Heme/DtxR_heme.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Heme/HrrA_heme.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Iron/DtxR_Iron.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Iron/HrrA_Iron.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Starvation/DtxR_Starvation.7z filter=lfs diff=lfs merge=lfs -text
+assays/2-1_Sequencing[[:space:]]and[[:space:]]Analysis/dataset/RawReads/Starvation/HrrA_Starvation.7z filter=lfs diff=lfs merge=lfs -text

assays/2-1_Sequencing and Analysis/protocols/Sequencing.txt

+The isolated DNA fragments were used for library preparation and indexing with TruSeq DNA PCR-free sample preparation kit (Illumina, Chesterford, UK) according to manufacturer’s instructions, leaving out the DNA size selection steps. Libraries were quantified using KAPA library quant kit (Peqlab, Bonn, Germany) and normalized for pooling. These pooled libraries were sequenced using the MiSeq device (Illumina) (paired-end sequencing, read length: 2 x 150 bases). 
\ No newline at end of file