Skip to content
Snippets Groups Projects
Commit 006845ee authored by Sabrina Zander's avatar Sabrina Zander
Browse files

Update README.md

parent 13ff446d
No related branches found
No related tags found
No related merge requests found
Hide whitespace changes
Inline Side-by-side
Showing
with 27 additions and 24 deletions
README.md
+ 27
− 24
View file @ 006845ee
...@@ -38,71 +38,74 @@ The [Assays (A)](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tr ...@@ -38,71 +38,74 @@ The [Assays (A)](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tr
### Chapter 1: S1_Loss_of_Khd4_Causes_Defects_in_Polar_Growth_of_Infectious_Hyphae ### Chapter 1: S1_Loss_of_Khd4_Causes_Defects_in_Polar_Growth_of_Infectious_Hyphae
[S1_A1_microscopy_yeast](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S1_A1_microscopy_yeast?ref_type=heads) Figure S1 [S1_A1_microscopy_yeast](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S1_A1_microscopy_yeast?ref_type=heads) // **Figure S1**
[S1_A2_microscopy_hyphae](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S1_A2_microscopy_hyphae?ref_type=heads) Figure 1 [S1_A2_microscopy_hyphae](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S1_A2_microscopy_hyphae?ref_type=heads) // **Figure 1**
### Chapter 2: S2_Establishing_HyperTRIBE_for_Fungal_RBPs ### Chapter 2: S2_Establishing_HyperTRIBE_for_Fungal_RBPs
[S2_A1_microscopy_Rrm4_Ada_strains_DIC+Rrm4-GFP](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp) Figure S2C-F [S2_A1_microscopy_Rrm4_Ada_strains_DIC+Rrm4-GFP](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A1_microscopy_Rrm4-Ada_Rrm4-Gfp) // **Figure S2C-F**
[S2_A2_protein_expression [S2_A2_protein_expression
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A2_protein_expression) Figure S3 A ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A2_protein_expression) // **Figure S3 A**
[S2_A3_microscopy_Rfp_Gfp [S2_A3_microscopy_Rfp_Gfp
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A3_microscopy_Rfp_Gfp) Figure S3 B-C ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A3_microscopy_Rfp_Gfp) // **Figure S3 B-C**
[S2_A4_HyperTRIBE_Rrm4_analysis [S2_A4_HyperTRIBE_Rrm4_analysis
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A4_HyperTRIBE_Rrm4_analysis) Figure 2C, Figure S4 A-D ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A4_HyperTRIBE_Rrm4_analysis) // **Figure 2C-D, Figure S4**
[S2_A5_HyperTRIBE_Rrm4_iCLIP [S2_A5_HyperTRIBE_Rrm4_iCLIP
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP) Figure 2D, Figure S4 E-G ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S2_A5_HyperTRIBE_Rrm4_iCLIP) // **Figure 2C-D, Figure S4**
### Chapter 3: S3_Khd4_Interacts_with_mRNAs_Enriched_for_Its_Binding_Motif_AUACCC ### Chapter 3: S3_Khd4_Interacts_with_mRNAs_Enriched_for_Its_Binding_Motif_AUACCC
[S3_A1_microscopy_Khd4-Gfp [S3_A1_microscopy_Khd4-Gfp
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A1_microscopy_Khd4-Gfp) Figure S5 C-E ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A1_microscopy_Khd4-Gfp) // **Figure S5 C-E**
[S3_A2_Khd4-Ada-strains_protein_expression [S3_A2_Khd4-Ada-strains_protein_expression
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A2_Khd4-Ada-strains_protein_expression) Figure S5 F-G ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A2_Khd4-Ada-strains_protein_expression) // **Figure S5 F-G**
[S3_A3_HyperTRIBE_Khd4_workflow [S3_A3_HyperTRIBE_Khd4_workflow
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A3_HyperTRIBE_Khd4_workflow) Figure 3A, Figure S6A ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A3_HyperTRIBE_Khd4_workflow) // **Figure 3A, Figure S6A**
[S3_A4_HyperTRIBE_motif_analysis [S3_A4_HyperTRIBE_motif_analysis
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A4_HyperTRIBE_motif_analysis) Figure S6 B-E, Figure 3, Figure S7, Figure S8 A-D ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A4_HyperTRIBE_motif_analysis) // **Figure S6 B-E, Figure 3, Figure S7, Figure S8 A-D**
[S3_A5_GO-term](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A5_GO-term) Figure S8 E, Figure S9 [S3_A5_GO-term](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S3_A5_GO-term) // **Figure S8 E, Figure S9**
### Chapter 4: S4_Loss_of_Khd4_Increases_the_Abundance_of_mRNAs_with_AUACCC_Motif_in_Their_3UTR ### Chapter 4: S4_Loss_of_Khd4_Increases_the_Abundance_of_mRNAs_with_AUACCC_Motif_in_Their_3UTR
[S4_A1_RNAseq_data [S4_A1_RNAseq_data
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S4_A1_RNAseq_data) Figure 4 A-C, Fig S10A ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S4_A1_RNAseq_data) // **Figure 4 A-C, Fig S10A**
[S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae) Figure 4 A-C, Fig S10A [S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S4_A2_RNAseq_analysis_khd4D_vs_wt_hyphae) // **Figure 4 A-C, Fig S10A**
### Chapter 5: S5_AUACCC_in_the_3_UTR_Functions_as_a_Khd4-Dependent_mRNA_Stability_Element ### Chapter 5: S5_AUACCC_in_the_3_UTR_Functions_as_a_Khd4-Dependent_mRNA_Stability_Element
[S5_A1_microscopy_khd4-targets [S5_A1_microscopy_khd4-targets
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A1_microscopy_khd4-targets) Figure S10B ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A1_microscopy_khd4-targets) // **Figure S10B**
[S5_A2_Kat_protein_level [S5_A2_Kat_protein_level
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A2_Kat_protein_level?ref_type=heads) Figure S10C, Figure 4E ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A2_Kat_protein_level?ref_type=heads) // **Figure S10C, Figure 4E**
[S5_A3_Kat_mRNA_level [S5_A3_Kat_mRNA_level
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A3_Kat_mRNA_level?ref_type=heads) Figure 4F ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S5_A3_Kat_mRNA_level?ref_type=heads) // **Figure 4F**
### Chapter 6: S6_Khd4_Tightly_Regulates_the_Expression_of_Distinct_Targets_Involved_in_Membrane_Trafficking ### Chapter 6: S6_Khd4_Tightly_Regulates_the_Expression_of_Distinct_Targets_Involved_in_Membrane_Trafficking
[S6_A1_RNAseq_analysis_yeast_vs_hyphae [S6_A1_RNAseq_analysis_yeast_vs_hyphae
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae) Figure 5 A-C, Figure 6, Figure S13 A-B ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae) // **Figure 5 A-C, Figure 6, Figure S13 A-B**
[S6_A2_analysis_specific_targets [S6_A2_analysis_specific_targets
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A2_analysis_specific_targets) Figure 11 B-D ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A2_analysis_specific_targets) // **Figure 5 A-C, Figure S11 B-D**
[S6_A3_Kat-strains_microscopy [S6_A3_Kat-strains_microscopy
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A3_Kat-strains_microscopy) Figure 5D, Figure S11 F ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A3_Kat-strains_microscopy) // **Figure 5D, Figure S11 F**
[S6_A4_relative_fluorescence_level [S6_A4_relative_fluorescence_level
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A4_relative_fluorescence_level) Figure S11 G ](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A4_relative_fluorescence_level) // **Figure S11 G**
### Chapter 7: S7_Loss_of_Khd4_Causes_Dysregulation_of_Membrane-Trafficking_Factors_in_khd4D_Cells ### Chapter 7: S7_Loss_of_Khd4_Causes_Dysregulation_of_Membrane-Trafficking_Factors_in_khd4D_Cells
see S6_A1 and S6_A2 [S6_A1_RNAseq_analysis_yeast_vs_hyphae
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S6_A1_RNAseq_analysis_yeast_vs_hyphae) // **Figure 5 A-C, Figure 6, Figure S13 A-B**
[S7_A1_RNAseq_analysis_Wt_Khd4D_hyphae_specific_genes
](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S7_A1_RNAseq_analysis_Wt_Khd4D_hyphae_specific_genes?ref_type=heads) // **Figure 6, Figure S13 A-B**
### Chapter 8: S8_Loss_of_Khd4_Causes_Defects_in_Vacuole_Formation_and_Localization ### Chapter 8: S8_Loss_of_Khd4_Causes_Defects_in_Vacuole_Formation_and_Localization
[S8_A1_microscopy_FM4-64](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S8_A1_microscopy_FM4-64?ref_type=heads) Figure S13 C-D [S8_A1_microscopy_FM4-64](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S8_A1_microscopy_FM4-64?ref_type=heads) // **Figure S13 C-D**
[S8_A2_microscopy_CAMG](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S8_A2_microscopy_CMAG?ref_type=heads) Figure 7 [S8_A2_microscopy_CAMG](https://git.nfdi4plants.org/sankaran/Sankaranarayanan-2023/-/tree/master/assays/S8_A2_microscopy_CMAG?ref_type=heads) // **Figure 7**
## License ## License
......
0% or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment