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Commit d862894c authored by Sabrina Zander's avatar Sabrina Zander
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add protocols to S1_A1 and S1_A2

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For microscopic analysis, 20 ml of yeast cells were grown in CM medium (1% glucose) to an OD600 of 0.5
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All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA).
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For microscopic analysis, 20 ml of yeast cells were grown in CM medium (1% glucose) to an OD600 of 0.5 Hyphal cells were induced by shifting 20 ml cell culture from CM medium to NM medium (supplemented either with 1% glucose or 1% arabinose) for 6, 9, 10, and 12 hours.
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All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA).
The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.
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