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Usadellab
Quinoa_Chileanfieldtrial
Commits
049c0942
Commit
049c0942
authored
3 weeks ago
by
Kathryn Dumschott
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3 weeks ago
Stage: arc_json
Stage: quality_report_generator
Stage: quality_report
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assays/RelativeWaterContent/README.md
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Leaf relative water content
#### Leaf relative water content
\ No newline at end of file
-
An approximately 1 cm 3 section of leaf was sampled from each plot
and transferred to a pre-weighed Eppendorf tube.
-
The tubes were then reweighed, filled with MilliQ water
and left at 4 °C overnight.
-
The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed.
-
Samples were then placed back into the emptied
Eppendorf tubes and placed in an oven at approximately 70 °C overnight.
-
Once leaf sections were completely
dry, the tubes containing the leaf samples were reweighed.
-
The empty tube weight was subtracted from the
fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the
equation:
$$RWC =
\f
rac{fresh weight - dry weight}{turgid weight - dry weight} ~x ~100 $$
\ No newline at end of file
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Jonathan Bauer
@bauer
mentioned in commit
a63f6222
·
3 weeks ago
mentioned in commit
a63f6222
mentioned in commit a63f6222a9f545f1016dcac2fab4582316adf962
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