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Commit 049c0942 authored by Kathryn Dumschott's avatar Kathryn Dumschott
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Edit README.md

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Leaf relative water content #### Leaf relative water content
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- An approximately 1 cm 3 section of leaf was sampled from each plot
and transferred to a pre-weighed Eppendorf tube.
- The tubes were then reweighed, filled with MilliQ water
and left at 4 °C overnight.
- The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed.
- Samples were then placed back into the emptied
Eppendorf tubes and placed in an oven at approximately 70 °C overnight.
- Once leaf sections were completely
dry, the tubes containing the leaf samples were reweighed.
- The empty tube weight was subtracted from the
fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the
equation:
$$RWC = \frac{fresh weight - dry weight}{turgid weight - dry weight} ~x ~100 $$
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