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Commit b95f9522 authored by Angela Kranz's avatar Angela Kranz
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Update README.md

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# *Ribes nigrum* genome sequencing experiment # *Ribes nigrum* genome sequencing experiment
To generate a blackcurrant genome assembly, DNA of the cultivar *R. nigrum* Rosenthals Langtraubige was used. DNA was extracted from young leaves of a single adult plant. Sequencing was performed with Oxford Nanopore Technologies, PacBio HiFi and Illumina. To generate a blackcurrant genome assembly, DNA of the cultivar *R. nigrum* Rosenthals Langtraubige was used. DNA was extracted from young leaves of a single adult plant. Sequencing was performed with Oxford Nanopore Technologies, PacBio HiFi and Illumina.
## Study metadata ## Study metadata
This is an overview about the metadata for the *R. nigrum* genome sequencing experiment. The metadata can also be found in the [isa.study file](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/blob/main/studies/Ribes_nigrum_genome/isa.study.xlsx?ref_type=heads). This is an overview about the metadata for the *R. nigrum* genome sequencing experiment. The metadata can also be found in the [isa.study file](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/blob/main/studies/Ribes_nigrum_genome/isa.study.xlsx?ref_type=heads).
...@@ -14,4 +14,15 @@ This is an overview about the metadata for the *R. nigrum* genome sequencing exp ...@@ -14,4 +14,15 @@ This is an overview about the metadata for the *R. nigrum* genome sequencing exp
## Experimental set-up ## Experimental set-up
![](./resources/RibesNigrum_Greenhouse.jpeg) ![](./resources/RibesNigrum_Greenhouse.jpeg)
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## Related repositories and files
After harvesting of leaves, the following steps were performed:
[DNA isolation](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Library preparation](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Sequencing](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Genome assembly](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/runs/Ribes_nigrum_genome?ref_type=heads)
Genome analysis
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