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Commit 2cd49f09 authored by Pia Saake's avatar Pia Saake
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......@@ -4,7 +4,7 @@ All experiments, including the generation of CRISPR/Cas9 knock-out lines, were p
## Barley growth conditions (6 days old)
Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol$m2$s1). Root and shoot fresh weight as well as length of the barley plants were measured.
Seeds were germinated on wet filter paper at room temperature in the dark under sterile conditions for three days before transfer to sterile jars containing solid 1/10 plant nutrition medium (PNM), pH 5.7 and 0.4% Gelrite (Duchefa, Haarlem, the Netherlands). Seedlings were cultured for six days in a growth chamber under long-day conditions (day/night cycle of 16/8 h, 22 C/18 C, light intensity of 108 mmol m-2 s-1). Root and shoot fresh weight as well as length of the barley plants were measured.
## Barley growth conditions (28 days old)
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