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Commit 9d55e2c0 authored by Gesa Helmsorig's avatar Gesa Helmsorig
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updated readme

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**Test diurnal gene expression of circadian clock and flowering time genes in Bowman and Bowman-derived introgression lines.**
2-week old plants from the genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were sampled at Zeitgeber Time (ZT) 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22.
Gene expression of CO1, ELF3, FT1, GI, LHY, LUX1, LWD1, PHYB, PHYC, Ppd-H1, PRR1, PRR59, PRR73, PRR95 and VRN1 were tested.
**The following assays were applied:**
1. leaf_sampling
2. RNA_extraction
3. cDNA_synthesis
4. qRT-PCR
Results can be found in Figure 3 and in Supplemental Figure S3E.
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