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+studies/biparental_mapping_eam7/resources/50k_Illumina_Infinium_iSelect_SNP_Array.xlsx filter=lfs diff=lfs merge=lfs -text
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+studies/biparental_mapping_eam7/resources/MorexV3_Araport11_conversion.txt filter=lfs diff=lfs merge=lfs -text

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README.md

+# _early maturity 7_ promotes early flowering by controlling the light input into the circadian clock in barley 
+
+## Original Publication
+
+Gesa Helmsorig, Agatha Walla, Thea Rütjes, Gabriele Buchmann, Rebekka Schüller, Götz Hensel, Maria von Korff, early maturity 7 promotes early flowering by controlling the light input into the circadian clock in barley, Plant Physiology, Volume 194, Issue 2, February 2024, Pages 849–866
+
+https://doi.org/10.1093/plphys/kiad551
+
+## Abstract
+
+Breeding for variation in photoperiod response is crucial to adapt crop plants to various environments. Plants measure changes in day length by the circadian clock, an endogenous timekeeper that allows plants to anticipate changes in diurnal and seasonal light–dark cycles. Here, we describe the _early maturity 7 (eam7)_ locus in barley _(Hordeum vulgare)_, which interacts with _PHOTOPERIOD 1 (Ppd-H1)_ to cause early flowering under non-inductive short days. We identify _LIGHT-REGULATED WD 1 (LWD1)_ as a putative candidate to underlie the _eam7_ locus in barley as supported by genetic mapping and CRISPR-Cas9-generated _lwd1_ mutants. Mutations in _eam7_ cause a significant phase advance and a misregulation of core clock and clock output genes under diurnal conditions. Early flowering was linked to an upregulation of _Ppd-H1_ during the night and consequent induction of the florigen _FLOWERING LOCUS T1_ under short days. We propose that _EAM7_ controls photoperiodic flowering in barley by controlling the light input into the clock and diurnal expression patterns of the major photoperiod response gene _Ppd-H1._
+
+## License
+
+© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.
+
+This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
\ No newline at end of file

_publication/README.md

+# _early maturity 7_ promotes early flowering by controlling the light input into the circadian clock in barley 
+
+Source: publication and supplementary data downloaded from https://doi.org/10.1093/plphys/kiad551
\ No newline at end of file

assays/DNA_extraction/README.md

+## DNA_extraction
+
+**Assay used in studies:**
+- allelism_test
+- biparental_mapping_eam7
+- generating_lwd1_mutants
+
+**Info:**
+
+No datasets, as DNA concentration was not measured and DNA samples were used in downstream assays (plant_genotyping)
\ No newline at end of file

assays/MSA_dissection/README.md

+## MSA_dissection
+
+**Assay used in studies:**
+- MSA_dissection_eam7
\ No newline at end of file