Protocols from manuscript added to all the assays
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- assays/S5_A5_X-ray structure analysis of H-Pab1-MLLE/protocols/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx 0 additions, 0 deletions...ls/Crystallization of MLLE3 of Rrm4 and MLLE of Pab1.docx
- assays/S5_A6_Western blot analysis of GST pull-down experiments using G-Pab1-MLLE and PAM2 variants/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions...variants/protocols/GST Pulldown and Western blotting.docx
- assays/S5_A7_Multiple sequence alignment of Pab1-MLLE orthologs/protocols/Multiple Sequence alignment.docx 0 additions, 0 deletions...MLLE orthologs/protocols/Multiple Sequence alignment.docx
- assays/S6_A1_Multiple Sequence alignment of vps8 and Taf7 orthologs/protocols/Multiple Sequence alignment.docx 0 additions, 0 deletions...Taf7 orthologs/protocols/Multiple Sequence alignment.docx
- assays/S6_A2_Protein structure prediction using Alphafold2/protocols/Structure prediction, modelling and analysis.docx 0 additions, 0 deletions...otocols/Structure prediction, modelling and analysis.docx
- assays/S6_A3_Western blot analysis of GST pull-down experiments using predicted PAM2L variants/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions...variants/protocols/GST Pulldown and Western blotting.docx
- assays/S6_A4_Hyphal growth of strains expressing Rrm4-Kat and Vps8-Gfp variants in AB33 background/protocols/Haphal growth and Microscopy data analysis.docx 0 additions, 0 deletions...protocols/Haphal growth and Microscopy data analysis.docx
- assays/S6_A5_Quantification of hyphal growth of AB33 derivatives expressing Rrm4-Kat and Vps8-Gfp variants/protocols/Microscopy data analysis.docx 0 additions, 0 deletions...Vps8-Gfp variants/protocols/Microscopy data analysis.docx
- assays/S6_A6_Micrographs and Kymograph measurement of Rrm4-Kat movement in Vps8-Gfp variant hypha/protocols/Microscopy data analysis.docx 0 additions, 0 deletions...Gfp variant hypha/protocols/Microscopy data analysis.docx
- assays/S6_A7_Quantification of processive Rrm4-Kat signals_velocity and distance travelled in vps8-gfp variant hypha/protocols/Microscopy data analysis.docx 0 additions, 0 deletions...gfp variant hypha/protocols/Microscopy data analysis.docx
- assays/S6_A8_Quantification of Rrm4-Kat signal accumulated at the tip/protocols/Microscopy data analysis.docx 0 additions, 0 deletions...ulated at the tip/protocols/Microscopy data analysis.docx
- assays/S7_A1_Multiple sequence alignment of Mkrn1 orthologs/protocols/Multiple Sequence alignment.docx 0 additions, 0 deletions...krn1 orthologs/protocols/Multiple Sequence alignment.docx
- assays/S7_A2_Protein structure prediction of MKRN1 using Alphafold2/protocols/Structure prediction, modelling and analysis.docx 0 additions, 0 deletions...otocols/Structure prediction, modelling and analysis.docx
- assays/S7_A3_Western blot analysis of GST pull-down experiments using human PAM2L variants/protocols/GST Pulldown and Western blotting.docx 0 additions, 0 deletions...variants/protocols/GST Pulldown and Western blotting.docx
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