add protocol to S1_A2

assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md

+# Calcofluor white staining 
 To visualize basal septa and empty sections, 1 ml of cell culture was stained with 1 μl of Calcofluor white staining solution (2 mg/ml; CFW) directly before microscopy with a DAPI filter set
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assays/S1_A2_microscopy_hyphae/protocols/Growth_condition.md

+# Growth conditions
 For microscopic analysis, 20 ml of yeast cells were grown in CM medium (1% glucose) to an OD600 of 0.5 Hyphal cells were induced by shifting 20 ml cell culture from CM medium to NM medium (supplemented either with 1% glucose or 1% arabinose) for 6, 9, 10, and 12 hours. 
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assays/S1_A2_microscopy_hyphae/protocols/measurements.md

+# measurements 
+The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain.
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assays/S1_A2_microscopy_hyphae/protocols/microscopy.md

+# microscopy
 All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA).
 The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain.
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