Laminarin (final concentration ∼ 60 mM) was incubated with biotin-hydrazide (120 mM) and sodium cyanoborohydride (1 M) for 2 h at 65 °C. The product was purified on a PD MidiTrap G-10 column (GE Healthcare) according to the manufacturer’s description. Success of biotinylation was validated via mass spectrometry. The immunogenic capacity of biotinylated laminarin was confirmed via ROS burst assays.
