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Identification of nuclear pore proteins at plasmodesmata

This is the ARC resulting from the Start Here guide in the DataPLANT Knowledge Base.

MAdLand Project - Erika Csicsely, Oguz Top, Wolfgang Frank et al.

This ARC accompanies the publication in Plant Journal: https://doi.org/10.1111/tpj.17236

DICER-LIKE (DCL) proteins play a central role in plant small RNA (sRNA) biogenesis. The genome of the early land plant Marchantia polymorpha encodes four DCL proteins: MpDCL1a, MpDCL1b, MpDCL3, and MpDCL4. While MpDCL1a, MpDCL3 and MpDCL4 show high similarities to their orthologs in Physcomitrium patens and Arabidopsis thaliana, MpDCL1b shares only a limited homology with PpDCL1b, but it is very similar, in terms of functional domains, to orthologs in other moss and fern species. We generated Mpdclge mutant lines for all MpDCL genes with the CRISPR/Cas9 system and conducted phenotypic analyses under control, salt stress, and phytohormone treatments to uncover specific MpDCL functions. The mutants displayed severe developmental aberrations, altered responses to salt and phytohormones, and disturbed sexual organ development. By combining mRNA and sRNA analyses, we demonstrate that MpDCLs and their associated sRNAs play pivotal roles in regulating development, abiotic stress tolerance and phytohormone response in M. polymorpha. We identified MpDCL1a in microRNA biogenesis, MpDCL4 in trans-acting small interfering RNA generation, and MpDCL3 in the regulation of pathogen-related genes. Notably, salt sensitivity in M. polymorpha is dependent on MpDCL1b and Mpdcl1bge mutants display enhanced tolerance and reduced miRNA expression in response to salt stress. We propose that M. polymorpha employs specific mechanisms for regulating MpDCL1b associated miRNAs under high salinity conditions, potentially shared with other species harboring MpDCL1b homologs.

RNA seq from Solanum lycopersicoides plants, performed for the manuscript entitled "A Solanum lycopersicoides reference genome facilitates insights into tomato specialized metabolism and immunity."

Multi-Modal Rat MRI + Light Sheet Brain Dataset

Traits linked to natural variation of sulfur content in Arabidopsis thaliana

An example of a trial containing traits that have been scored multiple times over a period of time.

Algae cultures were grown under mixotrophic (TAP) and phototrophic (HMP) conditions. During 24h of 35°C/40°C heat treatment 'omics samples were taken.

Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.