Explore ARCs

PPD-H1 Improves Stress Resistance and Energy Metabolism to Boost Spike Fertility under High Ambient Temperatures

In the present study we aimed at the highest possible resolution of this network by combining analysis of transcriptome, proteome and subcellularly resolved metabolome of plants that were exposed to rising carbon dioxide concentrations over a time course of one week.

A prototypic ARC that implements all specification standards accordingly

Fungal pathogens depend on sophisticated gene expression programs for successful infection. A crucial component is RNA regulation mediated by RNA-binding proteins (RBPs). However, little is known about the spatiotemporal RNA control mechanisms during fungal pathogenicity. Here, we discover that the RBP Khd4 defines a distinct mRNA regulon to orchestrate membrane trafficking during pathogenic development of Ustilago maydis. By establishing hyperTRIBE for fungal RBPs, we generated a comprehensive transcriptome-wide map of Khd4 interactions in vivo. We identify a defined set of target mRNAs enriched for regulatory proteins involved, e.g., in GTPase signaling. Khd4 controls the stability of target mRNAs via its cognate regulatory element AUACCC present in their 3′ untranslated regions. Studying individual examples reveals a unique link between Khd4 and vacuole maturation. Thus, we uncover a distinct role for an RNA stability factor defining a specific mRNA regulon for membrane trafficking during pathogenicity.

Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.

RNA seq from Solanum lycopersicoides plants, performed for the manuscript entitled "A Solanum lycopersicoides reference genome facilitates insights into tomato specialized metabolism and immunity."

An example of a trial containing traits that have been scored multiple times over a period of time.

Identification of nuclear pore proteins at plasmodesmata

Cofunctioning of bacterial exometabolites drives root microbiota establishment

Mapping the castor bean endosperm proteome revealed a metabolic interaction between plastid, mitochondria, and peroxisomes to optimize seedling growth

Identification of nuclear pore proteins at plasmodesmata