add strains S1

assays/S1_A2_microscopy_hyphae/protocols/Calcofluor white staining .md

+To visualize basal septa and empty sections, 1 ml of cell culture was stained with 1 μl of Calcofluor white staining solution (2 mg/ml; CFW) directly before microscopy with a DAPI filter set
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assays/S1_A2_microscopy_hyphae/protocols/microscopy.md

 All images were acquired using laser-based epifluorescence-microscopy, Zeiss Axio Observer.Z1 (Oberkochen, Germany) and processed using the MetaMorph software package (version 7.7.0.0, Molecular Devices, Seattle, IL, USA).
-The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.
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+The hyphal length was determined by measuring the distance between the tip and the basal pole without including the empty sections. Hyphal width was calculated by taking the average thickness measured at three different locations within each hypha.Hyphal cells with empty sections were visualized using Calcofluor White (CFW) and were scored manually. For each experiment, more than 100 cells were counted per strain.
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